L7773

Lysozyme from chicken egg white

aseptically filled

• 동의어(들): Mucopeptide N-acetylmuramoylhydrolase, Muramidase
• CAS Number: 12650-88-3
• 효소 위원회 번호: 3.2.1.17 (BRENDA, IUBMB)
• EC Number: 235-747-3
• MDL number: MFCD00131557
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 무균: aseptically filled
• Quality Level: 300
• 형태: powder
• enzyme activity: ≥40000 units/mg protein
• 분자량: single-chain 14.3 kDa
• UniProt 수납 번호: P00698
• 저장 온도: −20°C
• 유전자 정보: chicken ... LYZ(396218)

설명

애플리케이션

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

생화학적/생리학적 작용

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).

단위 정의

One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.

제조 메모

Prepared from L6876

기타 정보

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)