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Merck
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Key Documents

TR-1008

Sigma-Aldrich

Sodium Butyrate Solution

This reagent enhances the transient expression of plasmids containing the SV40 early promoter in transfected cells.

동의어(들):

Cell Culture NaBu

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About This Item

UNSPSC 코드:
12352207
eCl@ss:
32160801
NACRES:
NA.75

Quality Level

형태

liquid

제조업체/상표

Specialty Media

입력

sample type: mouse embryonic stem cell(s)

일반 설명

This reagent enhances the transient expression of plasmids containing the SV40 early promoter in transfected cells. Available as a 500mM stock solution (1mL). A complete protocol is included with shipment.

애플리케이션

Sodium butyrate can act to increase the efficiency of transfection and expression for both transient and stable transfections. It increases enhancer-dependant transcription of inserts driven by the SV40 early promoter. Vectors of this type include any of the pSV and pSV2 plasmids. Treatment of transfected cells with sodium butyrate can result in: a 2-3 fold increase in the number of cells able to express the insert; a 10-30 fold increase in the number of stable transfected colonies; and cells treated with sodium butyrate at the time of transfection can be re-induced by a second treatment weeks after the initial treatment and yield a 4-7 fold increase in transcription of the SV40 early promoter driven insert.



This treatment can be used on cells transfected by the Calcium Phosphate, DEAE Dextran and electroportation method.

저장 및 안정성

Store at 2-8°C for 2 years.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

픽토그램

Corrosion

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Storage Class Code

8B - Non-combustible corrosive hazardous materials

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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