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Merck
모든 사진(1)

Key Documents

I3390

Sigma-Aldrich

IMDM

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

동의어(들):

Iscove′s MDM, Iscoves MDM, Iscove’s Modified Dulbecco’s Medium, IMDM

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About This Item

MDL number:
UNSPSC 코드:
12352207
NACRES:
NA.75

product name

Iscove′s Modified Dulbecco′s Medium, liquid, sterile-filtered, With sodium bicarbonate, without L-glutamine, suitable for cell culture, suitable for hybridoma

Quality Level

무균

sterile-filtered

형태

liquid

기술

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

불순물

endotoxin, tested

성분

phenol red: yes
L-glutamine: no
glucose: yes

배송 상태

ambient

저장 온도

2-8°C

유사한 제품을 찾으십니까? 방문 제품 비교 안내

애플리케이션

Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove′s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

재구성

Supplement with 0.584 g/L L-glutamine

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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Albumin, transferrin, and lipids can replace serum entirely for support of LPS-stimulated murine B lymphocytes in culture. In the presence of these compounds, growth and maturation to IgM and IgG secretion, induced by lipopolysaccharide (LPS), occurs at the same or
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A murine hybridoma cell line (167.4G5.3) was cultured in batch mode using IMDM containing different serum concentrations and bovine serum albumin (BSA). Cell growth and death, metabolism and antibody production were studied in these cultures. The cells were more susceptible

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