추천 제품
product name
Phosphorylated Neurofilament (pNF-H) Sandwich ELISA Kit, The Phosphorylated Neurofilament H (pNF-H) kit is a sandwich enzyme immunoassay (EIA), which measures pNF-H.
Quality Level
종 반응성
pig, feline, rat, human, bovine, mouse
제조업체/상표
Chemicon®
assay range
sensitivity: 0.0293-15 ng/mL
(Range of Detection)
sensitivity: 0.0585 ng/mL
기술
ELISA: suitable
NCBI 수납 번호
UniProt 수납 번호
응용 분야
research use
검출 방법
colorimetric
배송 상태
dry ice
애플리케이션
Research Category
Neuroscience
Neuroscience
The Phosphorylated Neurofilament H (pNF-H) kit is a sandwich enzyme immunoassay (EIA), which measures pNF-H.
Used to detect/quantify: Phosphorylated Neurofilament (pNF-H)
포장
96 wells
성분
Immunoplate Pre-coated with Chicken anti-pNF-H Polyclonal Antibody: (Part No. 2006263). One 96-well immunoplate pre-coated with chicken anti- pNF-H polyclonal antibody sealed in a foil pouch.
Rabbit anti- pNF-H Polyclonal Antibody: (Part No. 2006919). One vial (100 μL) of rabbit anti- pNF-H polyclonal antibody.
Goat anti-Rabbit, Alkaline Phosphatase Conjugated Antibody: (Part No. 2006917). One vial (10 μL) of goat anti-rabbit alkaline phosphatase conjugate.
Bovine pNF-H Standard: (Part No. 2006918). One vial (5.0 ng) of bovine pNF-H standard.
20X TBS: (Part No. 2004913). One bottle (50 mL) of 20X TBS.
20% Tween-20® : (Part No. 2004915). One vial (3 mL) of 20% Tween-20.
Elisa Assay Diluent: (Part No. 2006921) One bottle (30 mL) of Elisa assay diluent containg 0.05% Kathon as a preservative, provided in a ready to use format.
pNPP Buffer: (Part No. 2006261). One bottle (12 mL) of pNPP buffer for use with 50X p-NPP, provided in a ready to use format.
50X pNPP: (Part No. 2006262). One vial (250 μL) of 50X pNPP (p-nitrophenyl phosphatase) alkaline phosphatase substrate.
Plate Covers - Two Plate Covers.
Rabbit anti- pNF-H Polyclonal Antibody: (Part No. 2006919). One vial (100 μL) of rabbit anti- pNF-H polyclonal antibody.
Goat anti-Rabbit, Alkaline Phosphatase Conjugated Antibody: (Part No. 2006917). One vial (10 μL) of goat anti-rabbit alkaline phosphatase conjugate.
Bovine pNF-H Standard: (Part No. 2006918). One vial (5.0 ng) of bovine pNF-H standard.
20X TBS: (Part No. 2004913). One bottle (50 mL) of 20X TBS.
20% Tween-20® : (Part No. 2004915). One vial (3 mL) of 20% Tween-20.
Elisa Assay Diluent: (Part No. 2006921) One bottle (30 mL) of Elisa assay diluent containg 0.05% Kathon as a preservative, provided in a ready to use format.
pNPP Buffer: (Part No. 2006261). One bottle (12 mL) of pNPP buffer for use with 50X p-NPP, provided in a ready to use format.
50X pNPP: (Part No. 2006262). One vial (250 μL) of 50X pNPP (p-nitrophenyl phosphatase) alkaline phosphatase substrate.
Plate Covers - Two Plate Covers.
저장 및 안정성
Prior to use maintain the kit components at 2°-8°C and -20°C, as indicated, until expiration date.
1. Wash Buffer - After dilution maintain the Wash Solution at 2°-8°C for up to one month or until expiration date.
2. Tween-20® - Store at room temperature upon receipt of kit.
3. Elisa Assay Buffer - Aliquot as required to avoid repeated freeze-thaw cycles. After thawing, maintain the Elisa Assay Buffer at 2°-8°C for up to one month, or until expiration date.
4. Immunoplate Pre-coated with Chicken anti- pNF-H Polyclonal Antibody - Unused strip wells may be returned to the foil pouch and stored at 2°-8°C for up to one month, or until expiration date.
1. Wash Buffer - After dilution maintain the Wash Solution at 2°-8°C for up to one month or until expiration date.
2. Tween-20® - Store at room temperature upon receipt of kit.
3. Elisa Assay Buffer - Aliquot as required to avoid repeated freeze-thaw cycles. After thawing, maintain the Elisa Assay Buffer at 2°-8°C for up to one month, or until expiration date.
4. Immunoplate Pre-coated with Chicken anti- pNF-H Polyclonal Antibody - Unused strip wells may be returned to the foil pouch and stored at 2°-8°C for up to one month, or until expiration date.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC
면책조항
For research use only. Not for use in diagnostic procedures.
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral
표적 기관
Kidney,Liver,Blood
Storage Class Code
10 - Combustible liquids
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Johannes Brettschneider et al.
Journal of neurology, 256(1), 3-12 (2009-03-10)
Guillain-Barré syndrome (GBS) is an acute inflammatory polyneuropathy affecting the myelin-protein sheathing and the axons themselves to various degrees. Damage to these structures causes biomarkers to be released into the adjacent body fluid compartment. In case of the proximal nerve
Axel Petzold et al.
Journal of immunological methods, 319(1-2), 34-40 (2006-12-05)
Recent studies suggest that the quantification of neurofilament subunits in cerebrospinal fluid (CSF), blood and amniotic fluid may reflect neuroaxonal damage and be of high clinical value. The present study aims to cross-validate two different independently developed ELISA techniques for
R Takano et al.
Neurology, 75(3), 208-216 (2010-07-21)
Loss of aquaporin 4 and glial fibrillary acidic protein (GFAP) with necrosis and demyelination is a prominent pathologic feature of neuromyelitis optica (NMO). However, the clinicopathologic significance of astrocytic damage and its relation with demyelination are unknown. To analyze clinical
The differential appearance of neurofilament triplet polypeptides in the developing rat optic nerve.
J S Pachter et al.
Developmental biology, 103(1), 200-210 (1984-05-01)
The ontogenetic appearance of the individual triplet polypeptides that comprise mammalian neurofilaments was studied in the developing rat optic nerve. Triton-insoluble cytoskeletal preparations from the optic nerves of rats of postnatal ages 1 Day (P1), 6 days (P6), 10 days
Gerry Shaw et al.
Biochemical and biophysical research communications, 336(4), 1268-1277 (2005-09-24)
Several lines of reasoning suggest that the phosphorylated axonal form of the neurofilament subunit NF-H is likely to be released from damaged and diseased neurons in significant amounts. Detection of this protein in serum or CSF might therefore provide information
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