MABN2728

Anti-FoxA2/HNF-3b Antibody, clone 4C7

• 동의어(들): Forkhead box A2, HNF-3 beta, FOXA2, Hepatocyte nuclear factor-3beta
• UNSPSC 코드: 12352203
• NACRES: NA.46

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 4C7, monoclonal
• 분자량: calculated mol wt 47.23 kDa; observed mol wt ~50 kDa
• 정제법: using protein G
• 종 반응성: human, chicken, mouse
• 포장: antibody small pack of 100
• 기술: electron microscopy: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 동형: IgG1κ
• 에피토프 서열: Unknown
• 단백질 ID 수납 번호: NP_990101
• UniProt 수납 번호: Q9PWP8
• 저장 온도: 2-8°C
• 유전자 정보: chicken ... HNF-3beta(395539)

설명

특이성

Clone 4C7 is a mouse monoclonal antibody that detects Hepatocyte nuclear factor-3β (HNF-3β/FoxA2).

면역원

Recombinant Chicken Rhodopsin (1-6).

애플리케이션

Quality Control Testing

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected FoxA2/HNF-3b, in HepG2 cell lysate.

Tested Applications

Electrophoretic Mobility Shift Assay: A representative lot detected FoxA2/HNF-3b in Electrophoretic Mobility Shift Assay (EMSA) (Foucher, I., et al. (2003). Development. 130(9): 1867-76).

Immunofluorescence Analysis: A representative lot detected FoxA2/HNF-3b in Immunofluorescence applications (Camp, D., et al. (2014). Development. 141(20): 3879-88; Hoelzl, M.A., et al. (2017). Dev Biol. 429(1): 132-146).

Immunoprecipitation Analysis: A representative lot immunoprecipitated FoxA2/HNF-3b in Immunoprecipitation applications (van den Brink, G.R., et al. (2001). Gastroenterology. 121(2):317-28).

Immunohistochemistry Applications: A representative lot detected FoxA2/HNF-3b in Immunohistochemistry applications (Ericson, J., et al. (1996). Cell. 87(4):661-73; Kwon, O., et al. (1998). J Clin Invest. 101(10):2054-64; Rousa, F.M., et al. (2000). Mol Cell Biol. 20(21):8264-82; van den Brink, G.R., et al. (2001). Gastroenterology. 121(2):317-28; Camp, D., et al. (2014). Development. 141(20):3879-88; Hoelzl, M.A., et al. (2017). Dev Biol. 429(1):132-146; Amarnath, S., et al. (2017). J Cell Sci. 130(1):119-131).

Immunocytochemistry Analysis: A representative lot detected FoxA2/HNF-3b in Immunohistochemistry applications (Nagase, T., et al. (2009). Dev Dyn. 238(5):1118-30).

Western Blotting Analysis: A representative lot detected FoxA2/HNF-3b in Western Blotting applications (Rousa, F.M., et al. (2000). Mol Cell Biol. 20(21):8264-82; van den Brink, G.R., et al. (2001). Gastroenterology. 121(2):317-28).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

표적 설명

Hepatocyte nuclear factor-3beta (UniProt: Q9PWP8; also known as HNF-3 beta, FOXA2) is encoded by the HNF-3beta gene (Gene ID: 395539) in Chicken. FoxA2 belongs to the forkhead family of transcription factors that uses a modified helix-turn-helix motif to bind DNA as a monomer. Its DNA binding domain is localized in amino acids 151-245. It is expressed very early during development and is involved in the development of several tissues, including liver, pancreas, lung, prostate, and the nervous system. In mouse embryo, FoxA2 expression is initiated during gastrulation in the node, notochord, and floor plate. Its induction in neural plate cells is an early response to notochord-derived signals and its ectopic expression induces floor plate differentiation. In the ventral mesencephalon and cerebellum, it is co-expressed with Engrailed homeoproteins - En1 and En2, which raises the possibility that the two transcription factors interact to regulate common target genes. FoxA2 expression is observed in different regions of the developing nervous system. Along with FoxA1, it is reported to be crucial for the specification and differentiation of dopamine neurons during embryonic development. FoxA2 also functions as a suppressor of tumor metastasis by inhibition of epithelial-to-mesenchymal transition in human lung cancers. It is shown to be a key target of TGF-β1 in controlling epithelial-to-mesenchymal transition (EMT) in lung cancer cells. (Ref.: Rausa, FM., et al. (2020). Mol. Cell. Biol. 20(21); 8264-8282; Domanskyi, A., et al (2014). Front. Cell. Neurosci. 8; 275; Tang, Y., et al. (2011). Cell Res. 21(2); 316-326; Foucher, I., et al. (2003). Development 130(9); 1867-1876; Ericson, J., et al. (1996). Cell. 87(4); 661-673).

물리적 형태

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

재구성

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Recommended storage: +2°C to +8°C.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable