추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
MS110, monoclonal
종 반응성
human
기술
immunocytochemistry: suitable
western blot: suitable
동형
IgG1κ
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... BRCA1(672)
일반 설명
Breast cancer type 1 susceptibility protein (BCRA1; RING finger protein 53) functions as a tumor suppressor. BRCA1 is an important member of the DNA repair pathway. After DNA damage, BRCA1 is phosphorylated on multiple serine residues by the ATR kinase or Chk2, and is localized to lesion sites with PCNA. BRCA1 interacts with a wide range of proteins including Rad50, NBS1, and Mre11, c-Abl tyrosine kinase, and γH2A.X, involved in the detection of damaged DNA and activation of appropriate repair pathways. BRCA1 may also respond to DNA damage by promoting gene expression through association with transcriptional proteins and RNA polymerase II, and may regulate the p53 pathway by this mechanism. Previous studies have indicated that mutations in the BRCA1 gene may contribute to the development of breast and ovarian cancer.
특이성
This antibody recognizes the N-terminus of BRCA1.
면역원
Epitope: N-terminus
Recombinant protein corresponding to the N-terminus of human BRCA1.
애플리케이션
Anti-BRCA1 Antibody, clone MS110 is an antibody against BRCA1 for use in Western Blotting, ICC.
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected in BRCA1 in MCF7 cells.
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated BRCA1 in IP (Wilson, C. A., et al. (1999). Nat Genet. 21(2):236-240.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected BRCA in ovarian cancer and breast cancer tissues (Scully, R., et al. (1996). Science. 272(5258):123-126; Wilson, C. A., et al. (1999). Nat Genet. 21(2):236-240.).
Immunofluorescence Analysis: A representative lot from an independent laboratory detected BRCA1 in a variety of certain cell lines (Scully, R., et al. (1996). Science. 272(5258):123-126.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated BRCA1 in IP (Wilson, C. A., et al. (1999). Nat Genet. 21(2):236-240.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected BRCA in ovarian cancer and breast cancer tissues (Scully, R., et al. (1996). Science. 272(5258):123-126; Wilson, C. A., et al. (1999). Nat Genet. 21(2):236-240.).
Immunofluorescence Analysis: A representative lot from an independent laboratory detected BRCA1 in a variety of certain cell lines (Scully, R., et al. (1996). Science. 272(5258):123-126.).
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
품질
Evaluated by Western Blot in HeLa nuclear extract.
Western Blot Analysis: 1 µg/mL of this antibody detected BRCA1 in 10 µg of HeLa nuclear extract.
Western Blot Analysis: 1 µg/mL of this antibody detected BRCA1 in 10 µg of HeLa nuclear extract.
표적 설명
~220 kDa and ~85 kDa observed. Multiple isoforms exist at various molecular weights and may be subject to post-translational modification.
물리적 형태
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
저장 및 안정성
Stable for 1 year at 2-8°C from date of receipt.
분석 메모
Control
HeLa nuclear extract
HeLa nuclear extract
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Lih-Ching Hsu
Biochemical and biophysical research communications, 360(2), 507-512 (2007-07-03)
The phosphorylation state of the tumor suppressor protein BRCA1 is tightly associated with its functions including cell cycle control and DNA repair. Protein kinases involved in the DNA damage checkpoint control, such as ATM, ATR, and hCds1/Chk2, have been shown
Filipe C Martins et al.
Cancer discovery, 2(6), 503-511 (2012-05-26)
BRCA1-associated breast tumors display loss of BRCA1 and frequent somatic mutations of PTEN and TP53. Here we describe the analysis of BRCA1, PTEN, and p53 at the single cell level in 55 BRCA1-associated breast tumors and computational methods to predict
Aaron Seo et al.
Proceedings of the National Academy of Sciences of the United States of America, 115(20), 5241-5246 (2018-05-02)
BRCA1 is essential for repair of DNA double-strand breaks by homologous recombination, and hence for survival. Complete loss of its function is lethal during early embryonic development. Patients who are compound heterozygous for BRCA1 truncating mutations and missense alleles that
Carly E Winton et al.
NPJ breast cancer, 2 (2016-09-02)
Recent advances in the development of functional materials offer new tools to dissect human health and disease mechanisms. The use of tunable surfaces is especially appealing as substrates can be tailored to fit applications involving specific cell types or tissues.
Localization of human BRCA1 and its loss in high-grade, non-inherited breast carcinomas.
Wilson, C A, et al.
Nature Genetics, 21, 236-240 (1999)
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