추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
2B, monoclonal
형태
liquid
포함
≤0.1% sodium azide as preservative
종 반응성
human
제조업체/상표
Calbiochem®
저장 조건
OK to freeze
동형
IgG2b
배송 상태
wet ice
저장 온도
−20°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... BRCA2(675)
일반 설명
Anti-BRCA2 (Ab-1), mouse monoclonal, clone 2B, recognzies the ~460 kDa BRCA2 protein in cells expressing a wild type BRCA2 gene. It is validated for Western blotting and immunoprecipitation.
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with myeloma cells. Recognizes the ~460 kDa BRCA2 protein.
Recognzies the ~460 kDa BRCA2 protein in cells expressing a wild type BRCA2 gene. Sold under license of U.S. Patent 5,753,441 and 6,162,897.
면역원
Human
a recombinant protein consisting of amino acids 1651-1821 of human BRCA2 fused to GST
애플리케이션
Immunoblotting (2 µg/ml)
Immunoprecipitation (2 µg)
Immunoprecipitation (2 µg)
포장
Please refer to vial label for lot-specific concentration.
경고
Toxicity: Standard Handling (A)
물리적 형태
In PBS.
재구성
Following initial thaw, aliquot and freeze (-20°C).
분석 메모
Positive Control
MCF7 cells
MCF7 cells
기타 정보
Andres, J.L., et al. 1998. Oncogene16, 2229.
Chen, J., et al. 1998. Mol. Cell2, 317.
Chen, P.L., et al. 1998. Proc. Natl. Acad. Sci. USA95, 5287.
Marmorstein, L.Y., et al. 1998. Proc. Natl. Acad. Sci. USA95, 13869.
Connor, F., et al. 1997. Nat. Genet.17, 423.
Sharan, S.K., et al. 1997. Nature386, 804.
Ludwig, T., et al. 1997. Genes Dev.11, 1226.
Mizuta, R., et al. 1997. Proc. Natl. Acad. Sci. USA94, 6927.
Rajan, J.V., et al. 1997. Dev. Biol.184, 385.
Suzuki, A., et al. 1997. Genes Dev.11, 1242.
Wong, A.K.C., et al. 1997. J. Biol. Chem.272, 31941.
Cannon-Albright, L.A. and M.H. Skolnick. 1996. Semin. Oncol.23, 1.
Goggins, M., et al. 1996. Cancer Res.56, 5360.
Stratton, M.R. 1996. Hum. Mol. Genet.5, 1515.
Tavtigian, S.V., et al. 1996. Nat. Genet.12, 333.
Rajan, J.V., et al. 1996. Proc. Natl. Acad. Sci. USA93, 13078.
Spillman, M.A. and A.M. Bowcock. 1996. Oncogene13, 1639.
Vaughn, J.P., et al. 1996. Cancer Res.56, 4590.
Wooster, R., et al. 1995. Nature378, 789.
Chen, J., et al. 1998. Mol. Cell2, 317.
Chen, P.L., et al. 1998. Proc. Natl. Acad. Sci. USA95, 5287.
Marmorstein, L.Y., et al. 1998. Proc. Natl. Acad. Sci. USA95, 13869.
Connor, F., et al. 1997. Nat. Genet.17, 423.
Sharan, S.K., et al. 1997. Nature386, 804.
Ludwig, T., et al. 1997. Genes Dev.11, 1226.
Mizuta, R., et al. 1997. Proc. Natl. Acad. Sci. USA94, 6927.
Rajan, J.V., et al. 1997. Dev. Biol.184, 385.
Suzuki, A., et al. 1997. Genes Dev.11, 1242.
Wong, A.K.C., et al. 1997. J. Biol. Chem.272, 31941.
Cannon-Albright, L.A. and M.H. Skolnick. 1996. Semin. Oncol.23, 1.
Goggins, M., et al. 1996. Cancer Res.56, 5360.
Stratton, M.R. 1996. Hum. Mol. Genet.5, 1515.
Tavtigian, S.V., et al. 1996. Nat. Genet.12, 333.
Rajan, J.V., et al. 1996. Proc. Natl. Acad. Sci. USA93, 13078.
Spillman, M.A. and A.M. Bowcock. 1996. Oncogene13, 1639.
Vaughn, J.P., et al. 1996. Cancer Res.56, 4590.
Wooster, R., et al. 1995. Nature378, 789.
The CAPAN-1 cell line, which has 6174delT mutation in the single BRCA2 allele, expresses a truncated BRCA2 protein of 230 kDa that contains the Anti-BRCA2 epitope and retains RAD51 binding but has lost p53 binding. Full length BRCA2 protein will co-migrate on SDS-PAGE with the catalytic subunit of DNA PK which can be detected using Anti-DNA PK, Cat. No. PC127. Antibody should be titrated for optimal results in individual systems.
법적 정보
Sold under license of U.S. Patents 5,837,492 and 6,124,104.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
M Goggins et al.
Cancer research, 56(23), 5360-5364 (1996-12-01)
Germline mutations in BRCA2 predispose carriers to the development of breast, ovarian, and a variety of other cancers. The original localization of the BRCA2 gene was aided by its homozygous deletion in a pancreatic carcinoma; indeed, an excess of pancreatic
A K Wong et al.
The Journal of biological chemistry, 272(51), 31941-31944 (1998-01-24)
Recent work has shown that the murine BRCA2 tumor suppressor protein interacts with the murine RAD51 protein. This interaction suggests that BRCA2 participates in DNA repair. Residues 3196-3232 of the murine BRCA2 protein were shown to be involved in this
F Connor et al.
Nature genetics, 17(4), 423-430 (1997-12-17)
Germline mutation of the BRCA2 gene carries a high risk of developing breast cancer. To study the function of this gene, we generated a mutation in Brca2 in mice. Unlike other mutations in the Brca2 gene, which are lethal early
J V Rajan et al.
Developmental biology, 184(2), 385-401 (1997-04-15)
Germline mutations in the putative tumor suppressor gene, BRCA1, predispose women to dramatically elevated risks of breast cancer, while germline mutations in the structurally unrelated gene, BRCA2, predispose both men and women to breast cancer. Recent studies have suggested an
S V Tavtigian et al.
Nature genetics, 12(3), 333-337 (1996-03-01)
Breast carcinoma is the most common malignancy among women in developed countries. Because family history remains the strongest single predictor of breast cancer risk, attention has focused on the role of highly penetrant, dominantly inherited genes in cancer-prone kindreds (1).
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