BI0166

Activator Reagent 0.3M BMT in AcN

Biosynthesis Novabiochem^®

• UNSPSC 코드: 41116006

속성

• Quality Level: 200
• 제품 라인: Novabiochem^®
• 양식: liquid
• 반응 적합성: reaction type: Coupling Reactions; reagent type: catalyst
• 전이 온도: flash point 2 °C (Acetonitrile)
• 배송 상태: ambient
• 저장 온도: 2-30°C

설명

일반 설명

Activator Reagent 0.3M 5-benzylmercaptotetrazole (BMT) in acetonitrile (AcN) is also known as benzylthiotetrazole (BTT) or benzyl-1H-tetrazole). It is used as a phosphoramidite activator reagent for oligonucleotide synthesis.

애플리케이션

0.3M BMT in AcN can be used as a reagent in solid phase phosphorylation of the oligomers. The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the 5′hydroxyl group of the growing oligonucleotide chain. The high activation potential of this reagent may be due to its greater acidity (pKa=4.08), which makes it easier to protonate the trivalent phosphorus in the initial stage of activation and increase the speed of the coupling reaction.

특징 및 장점

• efficient and rapid activation reagent that produces less
• phosphoramidite waste      
• higher solubility at lower temperatures      
• does not crystallize or clog lines       
• safer reagent to use

분석 메모

Color (APHA): 10APHA max
Component: Acetonitrile Purity (By GC): 99.9% min
Form: Clear Liquid
Identity (IR-spectrum): Conforms
Molarity of 5-Benzylmercaptotetrazole: 0.28-0.32M
Water (H2O): 1-30ppm

법적 정보

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• 픽토그램: GHS02,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H225,H302 + H312 + H332,H319
• 예방조치 성명서: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 3
• Flash Point (°F): 35.6 °F - closed cup - (Acetonitrile)
• Flash Point (°C): 2 °C - closed cup - (Acetonitrile)