AB5808

Anti-HCN4 Antibody

Chemicon^®, from rabbit

• 동의어(들): Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 4
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: rat, mouse, human
• 제조업체/상표: Chemicon^®
• 기술: immunohistochemistry: suitable; western blot: suitable
• NCBI 수납 번호: NM_005477.1
• UniProt 수납 번호: Q9Y3Q4
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... HCN4(10021)

설명

특이성

Recognizes HCN4 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 4). The epitope does not share homology with any other known proteins.

면역원

GST fusion protein corresponding to amino acids 119-155 of human HCN4 (Accession XP_007730, NP_005468, CAB42604, CAB52754).

애플리케이션

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

This Anti-HCN4 Antibody is validated for use in IH, WB for the detection of HCN4.

Western blot: 1:200 using ECL on rat brain membranes.

Immunohistochemistry using rat brain sections.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

물리적 형태

Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

저장 및 안정성

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

분석 메모

Control
CONTROL ANTIGEN: Included free of charge with the antibody is 120 μg of control antigen. The stock solution of the antigen can be made up using 100 μL of PBS. For positive control, in Western blot using 20 ng of protein per Minigel lane. For negative control, preincubate 3 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• 유해 및 위험 성명서: H412
• 예방조치 성명서: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3