481409

PTEN, His-Tag^® and S-Tag, Human Recombinant, S. frugiperda

• 동의어(들): Phosphatase and Tensin Homolog Deleted on Chromosome 10
• UNSPSC 코드: 12352202

속성

• 분석: ≥80% (SDS-PAGE)
• Quality Level: 100
• 형태: liquid
• 특이 활성도: ≥75 units/mg protein
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze
• 배송 상태: wet ice
• 저장 온도: −70°C

설명

일반 설명

Full-length, recombinant, human PTEN (GenBank target symbol = PTEN, ACC No. BC_005821) fused at N-terminus to a His•Tag and S•Tag sequences and expressed in S. frugiperda insect cells. PTEN is a tumor suppressor that is frequently found mutated in human cancers. It serves as a lipid phosphatase and negative regulator of the PI-3K/Akt pathway. Absence of functional PTEN results in constitutive activation of Akt and mTOR kinases.

포장

Please refer to vial label for lot-specific concentration.

경고

Toxicity: Standard Handling (A)

단위 정의

One unit is defined as the amount of enzyme required to liberate 1 nmol phosphate from PIP₃ per min at 37°C.

물리적 형태

In 150 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 1 mM EDTA, 50% glycerol, pH 7.5.

재구성

Following initial thaw, aliquot and freeze (-70°C).

기타 정보

Harrington, L.S., et al. 2005. Trends Biochem. Sci.30, 35.
Osaki, M., et al. 2004. Apoptosis9, 667.
Lennon, G., et al. 1996. Genomics33, 151.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable