203900

Bufalin

A cardiotonic steroid isolated from toads of Bufo species that potently inhibits ouabain-sensitive Na⁺,K⁺-ATPase activity (IC₅₀ = 1.4 nM).

• 동의어(들): Bufalin, 5β, 20(22)-Bufadienolide-3β, 14-diol
• 실험식(Hill 표기법): C₂₄H₃₄O₄
• CAS Number: 465-21-4
• Molecular Weight: 386.52
• UNSPSC 코드: 12352211
• NACRES: NA.77

속성

• Quality Level: 100
• 분석: ≥98% (HPLC)
• 양식: solid
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; protect from light
• 색상: white to off-white
• solubility: DMSO: 25 mg/mL; warm ethanol: 25 mg/mL
• 배송 상태: ambient
• 저장 온도: −20°C
• InChI: 1S/C24H34O4/c1-22-10-7-17(25)13-16(22)4-5-20-19(22)8-11-23(2)18(9-12-24(20,23)27)15-3-6-21(26)28-14-15/h3,6,14,16-20,25,27H,4-5,7-13H2,1-2H3/t16-,17+,18-,19?,20?,22+,23-,24+/m1/s1
• InChI key: QEEBRPGZBVVINN-ZXRSHIDQSA-N

설명

일반 설명

A cardiotonic steroid isolated from Bufo species toads that potently inhibits ouabain-sensitive Na⁺,K⁺-ATPase activity. Induces apoptosis in various human cell lines such as leukemia HL60 and U937 cells by altering the expression of apoptosis-related genes. Reported to stimulate the activities of p21 activated kinase (PAK) and c-Jun Kinase (JNK) in leukemic cell lines. Induces a transient increase in the expression of Tiam1 mRNA in U937 cell lines (~100 nM). Bufalin also down-regulates gene expression of the intracellular signaling protein 14-3-3 in rat lens, probably a consequence of Na⁺-K⁺-ATPase inhibitors. Reduces the level of topoisomerase II in human leukemia cells.

A cardiotonic steroid isolated from toads of Bufo species that potently inhibits ouabain-sensitive Na⁺,K⁺-ATPase activity (IC₅₀ = 1.4 nM). Induces apoptosis in various human cell lines such as leukemia HL-60 and U937 cells by altering the expression of apoptosis-related genes. Reported to stimulate the activities of p21 activated kinase (PAK) and c-Jun Kinase (JNK) in leukemic cell lines. Induces a transient increase in the expression of Tiam1 mRNA in U937 cell lines (~100 nM). Bufalin also down-regulates gene expression of the intracellular signaling protein 14-3-3 in rat lens, probably a consequence of Na⁺,K⁺-ATPase inhibition. Reduces the level of topoisomerase II in human leukemia cells.

생화학적/생리학적 작용

Cell permeable: no

Primary Target
Ouabain-sensitive Na+,K+-ATPase activity

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 1.4 nM against ouabain-sensitive Na+,K+-ATPase activity

포장

Packaged under inert gas

경고

Toxicity: Highly Toxic (H)

재구성

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

기타 정보

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Kurosawa, M., et al. 2000. Am. J. Physiol.278, C500.
Kawazoe, N., et al. 1999. Oncogene 18, 2413.
McGowan, M.H., et al. 1999. J. Pharmacol. Exp. Ther. 289, 1559.
Watabe, M., et al. 1998. Oncogene 16, 779.
Hashimoto, S., et al. 1997. Leuk. Res. 21, 875.
Morris, J.F., et al. 1997. Comp. Biochem. Physiol. A Physiol. 118, 599.
Brownlee, A.A., et al. 1990. Clin. Sci.78, 169.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• 픽토그램: GHS06
• 신호어: Danger
• 유해 및 위험 성명서: H300
• 예방조치 성명서: P264 - P270 - P301 + P310 - P405 - P501
• Hazard Classifications: Acute Tox. 2 Oral
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable