QBD10774

NHS-dPEG^®₂₄-biotin

• 동의어(들): Polyethylene glycol
• 실험식(Hill 표기법): C₆₅H₁₂₀N₄O₃₀S
• Molecular Weight: 1469.72
• UNSPSC 코드: 51171641
• PubChem Substance ID: 329823948
• NACRES: NA.22

속성

• 분석: >90%
• 양식: solid or viscous liquid
• 반응 적합성: reaction type: Biotinylations; reactivity: amine reactive; reagent type: cross-linking reagent
• 폴리머 구조: shape: linear; functionality: monofunctional
• 배송 상태: ambient
• 저장 온도: −20°C
• SMILES string: O=C(CCCCC1SCC(N2)C1NC2=O)NCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCC(ON3C(CCC3=O)=O)=O
• InChI: 1S/C65H120N4O30S/c70-60(4-2-1-3-59-64-58(57-100-59)67-65(74)68-64)66-8-10-76-12-14-78-16-18-80-20-22-82-24-26-84-28-30-86-32-34-88-36-38-90-40-42-92-44-46-94-48-50-96-52-54-98-56-55-97-53-51-95-49-47-93-45-43-91-41-39-89-37-35-87-33-31-85-29-27-83-25-23-8
• InChI key: FWKPYGZMJFADHK-UHFFFAOYSA-N

설명

일반 설명

NHS-dPEG^®₂₄-biotin is a cross-linking reagent used in biotinylation reactions. The N-hydroxysuccinimidyl (NHS) moiety of NHS-dPEG^®₂₄-biotin forms a stable amide bond with free primary amines, such as the amines on the side chain of lysine. The biotin moiety is used in a variety of applications, including labeling, supramolecular building, affinity chromatography, pull-down assays, and the production of biotinylated antibodies.

특징 및 장점

NHS-dPEG^®₂₄-biotin is used extensively to label molecules that contain a free amine with biotin. The single molecular weight NHS-dPEG^®₂₄-biotin compound has a long spacer of precise length (97.7 Å) between the biotin moiety and the labeled molecule. This dPEG^® spacer imparts a high degree of water solubility to the biotin label. For biotinylated peptides and proteins, the dPEG^® spacer dramatically reduces aggregation and precipitation of the modified molecule and reduces non-specific binding of the molecule, both of which are common problems with molecules that have been biotinylated with the widely used NHS-LC-biotin.

법적 정보

Products Protected under U.S. Patents # 7,888,536 B2

dPEG is a registered trademark of Quanta BioDesign

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable