40718

Diethyl pyrocarbonate

96% (NT)

• 동의어(들): DEP, DEPC, Diethyl dicarbonate, Diethyl oxydiformate, Ethoxyformic acid anhydride
• Linear Formula: O(COOC₂H₅)₂
• CAS Number: 1609-47-8
• Molecular Weight: 162.14
• Beilstein: 637031
• EC Number: 216-542-8
• MDL number: MFCD00009106
• UNSPSC 코드: 12352100
• PubChem Substance ID: 24865430
• NACRES: NA.22

속성

• Quality Level: 200
• 분석: 96% (NT)
• 형태: liquid
• 색상: APHA: ≤20
• refractive index: n20/D 1.398 (lit.); n20/D 1.398
• bp: 93-94 °C/18 mmHg (lit.)
• solubility: 95% ethanol: 4.5 g/10 mL, clear, colorless
• density: 1.121 g/mL at 20 °C; 1.101 g/mL at 25 °C (lit.)
• 배송 상태: wet ice
• 저장 온도: 2-8°C
• SMILES string: CCOC(=O)OC(=O)OCC
• InChI: 1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3
• InChI key: FFYPMLJYZAEMQB-UHFFFAOYSA-N

설명

일반 설명

Diethyl pyrocarbonate (DEP) is a bactericidal agent and decomposes in water to CO₂ and ethanol. DEP inhibits enzymes such as DNase and RNase without affecting nucleic acids. It is useful for the preparation of mRNA from tissues.

애플리케이션

Diethyl pyrocarbonate may be used in the following studies:

• For the modification of histidyl residues in proteins.
• As a nuclease inhibitor, for the extraction of undegraded nucleic acids from etiolated and green plant tissues.
• For the modification of linear and supercoiled plasmid DNAs.
• As a chemical probe to investigate the secondary structure in negatively supercoiled DNA.
• For cabethoxylation of histidine residues of actin.

Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.

생화학적/생리학적 작용

Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H302
• 예방조치 성명서: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): 156.2 °F - closed cup
• Flash Point (°C): 69 °C - closed cup
• 개인 보호 장비: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter