159220

Diethyl pyrocarbonate

96% (NT)

• 동의어(들): DEP, DEPC, Diethyl dicarbonate, Diethyl oxydiformate, Ethoxyformic acid anhydride
• Linear Formula: O(COOC₂H₅)₂
• CAS Number: 1609-47-8
• Molecular Weight: 162.14
• Beilstein: 637031
• EC Number: 216-542-8
• MDL number: MFCD00009106
• UNSPSC 코드: 12352100
• PubChem Substance ID: 24849799
• NACRES: NA.22

속성

• Quality Level: 200
• 분석: 96% (NT)
• 양식: liquid
• refractive index: n20/D 1.398 (lit.)
• bp: 93-94 °C/18 mmHg (lit.)
• density: 1.101 g/mL at 25 °C (lit.)
• 작용기: carbonate
• 배송 상태: wet ice
• 저장 온도: 2-8°C
• SMILES string: CCOC(=O)OC(=O)OCC
• InChI: 1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3
• InChI key: FFYPMLJYZAEMQB-UHFFFAOYSA-N

설명

일반 설명

Diethyl pyrocarbonate reacts selectively with the single-stranded loops of cruciform structures. The irreversible inactivation of ribonuclease with diethylpyrocarbonate has been studied.

애플리케이션

Diethyl pyrocarbonate was used as:

• nuclease inhibitor for the extraction of undegraded nucleic acids from etiolated and green plant tissues
• an antimicrobial additive
• ribonuclease inhibitor
• histidine residue modifier
• reagent for conversion of imines to carbamates

Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.

생화학적/생리학적 작용

Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H302
• 예방조치 성명서: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): 156.2 °F - closed cup
• Flash Point (°C): 69 °C - closed cup
• 개인 보호 장비: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter