- Production of protein kinase C-delta by the baculovirus-insect cell system in serum-supplemented and serum-free media.
Production of protein kinase C-delta by the baculovirus-insect cell system in serum-supplemented and serum-free media.
Journal of bioscience and bioengineering (2005-10-20)
Hideki Yamaji, Daigo Hirakawa, Shin-Ichi Tagai, Hideki Fukuda
PMID16233389
要旨
When rat protein kinase C-delta (PKC-delta) was produced by Sf9 cells infected with a recombinant baculovirus in shake-flask culture using a serum-containing medium, the intracellular PKC-delta content decreased in the late period while the extracellular PKC-6 markedly increased. During the late period of serum-free culture, the extracellular PKC-6 level considerably declined, but the addition of a protease inhibitor, leupeptin, prevented the reduction in PKC-delta production.
材料
製品番号
ブランド
製品内容
SAFC
EX-CELL® 420、昆虫細胞用無血清培地, with L-glutamine, sterile-filtered, suitable for insect cell culture
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