Oncogenic PIK3CA induces centrosome amplification and tolerance to genome doubling.

Oncogenic PIK3CA induces centrosome amplification and tolerance to genome doubling.

Nature communications (2017-11-25)

Inma M Berenjeno, Roberto Piñeiro, Sandra D Castillo, Wayne Pearce, Nicholas McGranahan, Sally M Dewhurst, Valerie Meniel, Nicolai J Birkbak, Evelyn Lau, Laurent Sansregret, Daniele Morelli, Nnennaya Kanu, Shankar Srinivas, Mariona Graupera, Victoria E R Parker, Karen G Montgomery, Larissa S Moniz, Cheryl L Scudamore, Wayne A Phillips, Robert K Semple, Alan Clarke, Charles Swanton, Bart Vanhaesebroeck

PMID29170395

要旨

Mutations in PIK3CA are very frequent in cancer and lead to sustained PI3K pathway activation. The impact of acute expression of mutant PIK3CA during early stages of malignancy is unknown. Using a mouse model to activate the Pik3ca

材料

製品番号

ブランド

製品内容

P8920

Sigma-Aldrich

ポリ-L-リシン溶液, 0.1 % (w/v) in H₂O

D9663

Sigma-Aldrich

ロバ血清

G9023

Sigma-Aldrich

ヤギ血清

H7904

Sigma-Aldrich

（Z）-4-ヒドロキシタモキシフェン, ≥98% Z isomer

05-636

Sigma-Aldrich

抗phospho-ヒストンH2A.X （Ser139）抗体、クローンJBW301, clone JBW301, Upstate^®, from mouse

A1978

Sigma-Aldrich

抗β-アクチン抗体, マウスモノクローナル, clone AC-15, purified from hybridoma cell culture

M1404

Sigma-Aldrich

ノコダゾール, ≥99% (TLC), powder

CS0030

Sigma-Aldrich

老化細胞組織化学染色キット, sufficient for 100 tests

T6074

Sigma-Aldrich

抗αチューブリン抗体、マウスモノクローナル, clone B-5-1-2, purified from hybridoma cell culture

R5503

Sigma-Aldrich

リボヌクレアーゼA ウシ膵臓由来, Type I-AS, 50-100 Kunitz units/mg protein

X100

Sigma-Aldrich

Triton^™ X-100, laboratory grade

T4424

Sigma-Aldrich

トリプシン溶液ブタ膵臓由来, 1 ×, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin per liter in Hanks′ Balanced Salt Solution with phenol red

A7906

Sigma-Aldrich

ウシ血清アルブミンウシ血清由来, heat shock fraction, pH 7, ≥98%

A5044

Sigma-Aldrich

モノクロナール抗α-アクチニンマウス宿主抗体, clone BM-75.2, ascites fluid

D1641

Sigma-Aldrich

ジヒドロサイトカラシンB