コンテンツへスキップ
Merck

Angiogenesis stimulated by novel nanoscale bioactive glasses.

Biomedical materials (Bristol, England) (2015-03-26)
Cong Mao, Xiaofeng Chen, Guohou Miao, Cai Lin
要旨

The ability of biomaterials to induce rapid vascular formation is critical in tissue regeneration. Combining recombinant angiogenic growth factors with bioengineered constructs have proven to be difficult due to several issues, including the instability of recombinant proteins, the need for sustained delivery and the dosage of factors. New formulations of bioactive glass, 58S nanosized bioactive glass (58S-NBG), have been reported to enhance wound healing in animal models better than the first generation of 45S5 Bioglass. Therefore, we investigated the effects of extracts of 58S-NBG and 80S-NBG on cultures of human umbilical vein endothelial cells (HUVECs). Cell viability was assessed by MTS assay. In vitro angiogenesis was measured using an ECM gel tube formation assay, and levels of mRNAs for five angiogenic related genes were measured by qRT-PCR. Extracts of 58S-NBG and 80S-NBG stimulated the proliferation of HUVECs, accelerated cell migration, up-regulated expression of the vascular endothelial growth factor, basic fibroblast growth factor, their receptors, and endothelial nitric oxide synthase, resulting in enhanced tube formation in vitro. The enhanced angiogenic response correlated with increased levels of Ca and Si in the extracts of 58S-NBG and 80S-NBG. The ability of 58S-NBG and 80S-NBG to stimulate angiogenesis in vitro provides alternative approaches for stimulating neovascularization of tissue-engineered constructs.

材料
製品番号
ブランド
製品内容

Sigma-Aldrich
エタノール, JIS special grade, ≥99.5%
Sigma-Aldrich
トリエチル ホスファート, ReagentPlus®, ≥99.8%
Sigma-Aldrich
1,3-ジアミノプロパン, ≥99%
Sigma-Aldrich
エタノール, SAJ first grade, ≥99.5%
Sigma-Aldrich
エチルアルコール(純粋), 190 proof, ACS spectrophotometric grade, 95.0%
Sigma-Aldrich
硝酸カルシウム 四水和物, suitable for cell culture, suitable for plant cell culture
Sigma-Aldrich
リン酸水素二アンモニウム 二塩基性, ≥99.99% trace metals basis
Supelco
エタノール標準品10% (v/v), 10 % (v/v) in H2O, analytical standard
Sigma-Aldrich
エタノール, ≥99.5%, suitable for HPLC
Sigma-Aldrich
エタノール, JIS special grade, 94.8-95.8%
Sigma-Aldrich
エタノール, ≥99.5%
Sigma-Aldrich
エタノール, ≥99.5%, suitable for absorption spectrum analysis
Sigma-Aldrich
Ethanol Fixative 80% v/v, suitable for fixing solution (blood films)
Sigma-Aldrich
エタノール, JIS first grade, 94.8-95.8%
Sigma-Aldrich
ビシンコニン酸 二ナトリウム塩 水和物, ≥98% (HPLC)
Sigma-Aldrich
硝酸カルシウム 四水和物, BioXtra, ≥99.0%
Sigma-Aldrich
エタノール, ≥99.5%, SAJ super special grade
Sigma-Aldrich
硝酸カルシウム 四水和物, JIS special grade, ≥98.5%
Sigma-Aldrich
エタノール, JIS 1000, ≥99.5%, for residue analysis
Sigma-Aldrich
エタノール, ≥99.5%, suitable for fluorescence
Sigma-Aldrich
エタノール, JIS 300, ≥99.5%, for residue analysis
Sigma-Aldrich
硝酸カルシウム 四水和物, SAJ first grade, ≥98.0%
Sigma-Aldrich
リン酸水素二アンモニウム 二塩基性, JIS special grade, ≥99.0%
Sigma-Aldrich
リン酸水素二アンモニウム 二塩基性, SAJ first grade, 98.5-101.0%