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Lipidic cubic phase serial millisecond crystallography using synchrotron radiation.

IUCrJ (2015-04-14)
Przemyslaw Nogly, Daniel James, Dingjie Wang, Thomas A White, Nadia Zatsepin, Anastasya Shilova, Garrett Nelson, Haiguang Liu, Linda Johansson, Michael Heymann, Kathrin Jaeger, Markus Metz, Cecilia Wickstrand, Wenting Wu, Petra Båth, Peter Berntsen, Dominik Oberthuer, Valerie Panneels, Vadim Cherezov, Henry Chapman, Gebhard Schertler, Richard Neutze, John Spence, Isabel Moraes, Manfred Burghammer, Joerg Standfuss, Uwe Weierstall
要旨

Lipidic cubic phases (LCPs) have emerged as successful matrixes for the crystallization of membrane proteins. Moreover, the viscous LCP also provides a highly effective delivery medium for serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs). Here, the adaptation of this technology to perform serial millisecond crystallography (SMX) at more widely available synchrotron microfocus beamlines is described. Compared with conventional microcrystallography, LCP-SMX eliminates the need for difficult handling of individual crystals and allows for data collection at room temperature. The technology is demonstrated by solving a structure of the light-driven proton-pump bacteriorhodopsin (bR) at a resolution of 2.4 Å. The room-temperature structure of bR is very similar to previous cryogenic structures but shows small yet distinct differences in the retinal ligand and proton-transfer pathway.

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製品内容

Sigma-Aldrich
リン酸カリウム 一塩基性, powder, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%
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リン酸カリウム 一塩基性, for molecular biology, ≥98.0%
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L-リシン 一塩酸塩, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%
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リン酸カリウム 一塩基性, BioUltra, for molecular biology, anhydrous, ≥99.5% (T)
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リン酸カリウム 一塩基性, 99.99% trace metals basis
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リン酸カリウム 一塩基性, ReagentPlus®
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リン酸カリウム 一塩基性, JIS special grade, ≥99.0%
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L-リシン 一塩酸塩, reagent grade, ≥98% (HPLC)
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リン酸カリウム 一塩基性, SAJ first grade, ≥99.0%
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L-リシン 一塩酸塩, BioUltra, ≥99.5% (AT)
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リン酸カリウム 一塩基性, ≥99.5%
Sigma-Aldrich
L-リシン 一塩酸塩, SAJ special grade, ≥99.0%