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Merck

Isolation and Characterization of Adult Cardiac Fibroblasts and Myofibroblasts.

Journal of visualized experiments : JoVE (2020-04-01)
Meiling Melzer, David Beier, Pampee P Young, Sarika Saraswati
要旨

Cardiac fibrosis in response to injury is a physiological response to wound healing. Efforts have been made to study and target fibroblast subtypes that mitigate fibrosis. However, fibroblast research has been hindered due to the lack of universally acceptable fibroblast markers to identify quiescent as well as activated fibroblasts. Fibroblasts are a heterogenous cell population, making them difficult to isolate and characterize. The presented protocol describes three different methods to enrich fibroblasts and myofibroblasts from uninjured and injured mouse hearts. Using a standard and reliable protocol to isolate fibroblasts will enable the study of their roles in homeostasis as well as fibrosis modulation.

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製品内容

Sigma-Aldrich
モノクロナール抗アクチン, α-平滑筋, clone 1A4, ascites fluid
Sigma-Aldrich
モノクロナール抗ビメンチン マウス宿主抗体, clone LN-6, ascites fluid
Sigma-Aldrich
抗FSP1/S100A4抗体, from rabbit