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Merck

Sonic hedgehog expression in the postnatal brain.

Biology open (2019-03-07)
Aileen Rivell, Ronald S Petralia, Ya-Xian Wang, Ellie Clawson, Keelin Moehl, Mark P Mattson, Pamela J Yao
要旨

Beyond its role in patterning the neural tube during embryogenesis, additional functions of Sonic hedgehog (Shh) in post-embryonic and mature brains have been coming into focus. However, the question of the abundance of endogenous Shh - the ligand of the signaling pathway - and its changes over time in post-embryonic and mature brains are less well understood. Here we find that while the amounts of Shh transcript and protein in rat brains are nearly undetectable at birth, they increase continuously during postnatal development and remain at readily detectable levels in young adults. This developmental age-associated increase in Shh levels is also seen in hippocampal neurons grown in culture, in which very young neurons produce minimal amounts of Shh protein but, as neurons grow and form synapses, the amounts of Shh increase significantly. Using immunolabeling with antibodies to different residues of Shh, we observed that the N-terminal fragment and the C-terminal fragment of Shh are present in hippocampal neurons, and that these two Shh forms co-exist in most compartments of the neuron. Our findings provide a better understanding of Shh expression in the brain, laying the groundwork for further comprehending the biogenesis of Shh protein in the young and mature brain and neurons.

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製品内容

Sigma-Aldrich
モノクロナール抗β-アクチン マウス宿主抗体, clone AC-15, ascites fluid
Sigma-Aldrich
抗アクチン ウサギ宿主抗体, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
シトシンβ-D-アラビノフラノシド, crystalline, ≥90% (HPLC)
Sigma-Aldrich
モノクロナール抗MAP2 マウス宿主抗体, clone HM-2, purified from hybridoma cell culture
Sigma-Aldrich
モノクロナール抗シナプトフィジン マウス宿主抗体, clone SVP-38, ascites fluid