G005753

HPLC Analysis of an Energy Drink on Ascentis^® Express HILIC (UV Detection)

application for HPLC

特性

• テクニック: HPLC: suitable
• 試験パラメータ: column: Ascentis Express HILlC, 10 cm x 3.0 mm I.D., 2.7 μm particles (53970-U); mobile phase: [A] 100 mM ammonium acetate, pH 5.0 with acetic acid; [B] water; [C] acetonitrile; (09:01:90, A:B:C); flow rate: 0.6 mL/min; pressure: 812 psi (56 bar); column temp.: 35 °C; detector: UV, 254 nm; injection: 2 μL; sample: Dilute 1:9 in acetonitrile
• 適合性: application for HPLC
• アプリケーション: food and beverages

詳細

アナリシスノート

This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of common drink additives used in caffeinated energy drinks. The analysis was performed with UVand ELSD detectors in series, this chromatogram being from the UV.

法的情報

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

アナライト

Caffeine
Benzoic acid
Sorbic acid