G005682

HPLC Analysis of Caffeine and Taurine on Ascentis^® Express HILIC Column using Evaporative Light Scattering Detection (ELSD)

application for HPLC

特性

• テクニック: HPLC: suitable
• 試験パラメータ: column: Ascentis Express HILlC, 5 cm x 3.0 mm I.D., 2.7 μm particles (53967-U); mobile phase: [A] 100 mM ammonium formate, pH 3.0 with formic acid; [B] acetonitrile; (10:90, A:B); flow rate: 0.6 mL/min; column temp.: 25 °C; detector: ELSD, 55 °C, 3.5 bar nitrogen; injection: 2 μL; sample: 1000 μg/mL in 10:90, water: acetonitrile
• 適合性: application for HPLC
• アプリケーション: vitamins, nutraceuticals, and natural products

詳細

アナリシスノート

This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of caffeine and taurine. Elution is by aqueous normal phase (ANP) as an increase in organic will increase retention.

法的情報

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

アナライト

Caffeine
Taurine