G004432

HPLCによるラット結晶中のスタチンの分析、Ascentis^® Express C18、SPE処理、Supel^™-Select HLB使用

application for SPE, application for LC-MS

特性

• テクニック: LC/MS: suitable; solid phase extraction (SPE): suitable
• 試験パラメータ: sample preparation: SPE (Solid Phase Extraction); sample/matrix: rat plasma spiked with provastin and atorvastin at 5 and 100 ng/mL; SPE tube/cartridge: Supel-Select HLB SPE, 30 mg/1 mL (54181-U); condition: 0.5 mL methanol:acetonitrile (1:1, v/v); 0.5 mL DI water; sample addition: 0.5 mL; washing: 0.5 mL 5% methanol; elution: 0.5 mL methanol:acetonitrile (1:1, v/v); column: Ascentis Express C18, 5 cm x 2.1 mm, 2.7 μm particles (53822-U); eluate post-treatment: evaporate to dryness with nitrogen at 37 °C, reconstitute in 0.5 mL DI water; mobile phase: 0.1% acetic acid diluted in 60% methanol; flow rate: 0.2 mL/min; column temp.: 35 °C; detector: ABI 3200 QT; ESI(+), MRM (423.3/321.3, 423.3/101.0, 557.3/397.2 and 557.3/453.4); injection: 5 μL
• 適合性: application for LC-MS; application for SPE
• アプリケーション: clinical

詳細

法的情報

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Supel is a trademark of Sigma-Aldrich Co. LLC