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製品名
TSKgel® Phenyl-5PW RP HPLC Column, phase phenyl, octadecyl, L × I.D. 7.5 cm × 8 mm, 10 μm particle size
物質
glass
製品種目
TSKgel®
メーカー/製品名
Tosoh 14007
標識化の程度
1 meq/mL ligand density (estimate)
パラメーター
no limitation salt concentration
0-100% organic solvent
2.5 mL/min flow rate
20 bar max. pressure
4-60 °C temperature
テクニック
HPLC: suitable
L × 内径
7.5 cm × 8 mm
Matrix
hydroxylated methacrylic polymer
マトリックス活性基
phenyl phase, octadecyl
粒径
10 μm
使用pH
2-12
分離法
reversed phase
詳細
Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of strong basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel® columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns.
- Octadecyl-2PW particles have 125 A pores
- Octadecyl-4PW particles have 500 Apores
- Phenyl-5PW RP particles have 1000 A pores
- Octadecyl-NPR particles are nonporous
物理的形状
Shipped in 30% methanol - 70% water.
法的情報
TSKgel is a registered trademark of Tosoh Corporation
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