UFC210024

Amicon^® Ultra Centrifugal Filter, 100 kDa MWCO

sample volume 2 mL, regenerated cellulose membrane, MWCO 100 kDa

• 別名: Centrifugal concentrator, 100 kDa MWCO, 2 mL sample volume
• UNSPSCコード: 41104916
• eCl@ss: 36100101
• NACRES: NB.24

特性

• 物質: polypropylene tube (for collection); regenerated cellulose membrane; styrene-butadiene housing
• 品質水準: 400
• 無菌性: non-sterile
• 製品種目: Amicon^®
• メーカー／製品名: Amicon^® Ultra
• パラメーター: 2 mL sample volume
• テクニック: protein extraction: suitable; protein purification: suitable
• L: 119.7 mm
• 直径: 15.9 mm
• ろ過面積: 1 cm²
• 容量: 2 mL
• ポアサイズ: 100 kDa MWCO
• 輸送温度: ambient

詳細

詳細

Amicon^® Ultra-2 mL centrifugal devices with Ultracel^® 100,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, IgG (156 kDa) at 1 mg/ml initial concentration is typically retained at 95% .
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 50-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
The device can be spun in a swinging bucket or fixed angle rotor. Amicon^® Ultra-2 mL devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon^® Ultra 2 mL filters deliver the highest recovery with shortest spin times.

アプリケーション

• Ultrafiltration with 100,000 Dalton molecular weight cutoff Ultracel^® membrane.
• Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
• Purification of macromolecular components found in tissue culture extracts or cell lysates.
• Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC.
• Desalting, buffer exchange and protein dialysis.
• Size exclusion and protein fractionation.

特徴および利点

Minimum Final Concentrate Volume: 15 µL–70 µL

関連事項

Replaces: 4211

物理的形状

Color Code: 透明

法的情報

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany

ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany