ZRB2053

Anti-SLC12A2 Antibody, clone 1J6 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 別名: Solute carrier family 12 member 2 (UniProt: P55011; also known as Basolateral Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 200
• リコンビナント: expressed in HEK 293 cells
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 1J6, recombinant monoclonal
• 詳細: 1J6 Clone
• 製品種目: ZooMAb^® learn more
• 形状: lyophilized
• 分子量: calculated mol wt 131.45 kDa; observed mol wt ~130 kDa
• 精製方法: using Protein A
• 化学種の反応性: human, mouse
• 化学種の反応性（ホモロジーによる予測）: rat, horse, porcine, monkey, feline, bovine, canine
• 包装: antibody small pack of 25 μL
• 環境により配慮した代替製品の特徴: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 強化検証: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• テクニック: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• アイソタイプ: IgG
• エピトープ配列: C-terminal cytoplasmic domain
• タンパク質IDアクセッション番号: NP_001037
• UniProtアクセッション番号: P55011
• 環境により配慮した代替製品カテゴリ: , Aligned
• 輸送温度: ambient
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... SLC12A2(6558)

詳細

詳細

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 1J6 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Solute carrier family 12 member 2 (SLC12A2). It targets an epitope within 19 amino acids from the C-terminal, cytoplasmic domain.

免疫原

KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal, cytoplasmic domain of human Solute carrier family 12 member 2 (SLC12A2).

アプリケーション

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SLC12A2 in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected SLC12A2 in Human and Mouse brain tissue lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SLC12A2 in human pancreas tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SLC12A2 in HeLa cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

ターゲットの説明

Solute carrier family 12 member 2 (UniProt: P55011; also known as Basolateral Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, SLC12A2) is encoded by the SLC12A2 (also known as NKCC1) gene (Gene ID: 6558) in human. SLC12A2 belongs to the subfamily of cation-chloride cotransporters that mediate electroneutral transport of sodium, potassium, and chloride across the plasma membrane. It is a multi-pass membrane protein with seven cytoplasmic domains, sixteen transmembrane domains (including two discontinuous), and six extracellular domains. It is expressed in many tissues and plays a vital role in the regulation of ionic balance and cell volume. Its activity is inhibited by bumetanide and furosemide-type of compounds. It has a major impact on intracellular chloride ion concentration in vascular smooth muscle cells (VSMC) and in neuronal cells. In primary hypertension, its activity is increased in VSMC and paraventricular nucleus neurons that leads to elevated peripheral resistance in systemic circulation and activation of sympathetic nervous system. Mutations in SLC12A2 gene have been linked to antenatal Bartter syndrome 1, an autosomal recessive disorder that is characterized by impaired salt reabsorption in the thick ascending loop of Henle, salt wasting, hypokalemic metabolic alkalosis, and hypercalcinuria. Some mutations are also associated with neurodevelopment disorders and hearing loss. Two isoforms of SLC12A2 have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Mutai, H., et al. (2020). PLoS Genet.16(4); e1008643; Stodberg, T., et al. (2020). Neurol Genet 6; :e478; Simon, DB., et al. (1996). Nat. Genet. 13(2); 183-188).

物理的形状

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

再構成

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

保管および安定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法的情報

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable