ZRB1721

Anti-EGR1 Antibody, clone 1F22 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 別名: Early growth response protein 1;AT225;Nerve growth factor-induced protein A;NGFI-A;Transcription factor ETR103;Transcription factor Zif268;Zinc finger protein 225;Zinc finger protein Krox-24
• UNSPSCコード: 12352203

特性

• 由来生物: rabbit
• 品質水準: 200
• リコンビナント: expressed in HEK 293 cells
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 1F22, recombinant monoclonal
• 詳細: recombinant, expressed in HEK 293 cells
• 製品種目: ZooMAb^® learn more
• 形状: lyophilized
• 分子量: calculated mol wt 57.51 kDa; observed mol wt ~80 kDa
• 精製方法: using Protein A
• 化学種の反応性: human
• 化学種の反応性（ホモロジーによる予測）: monkey
• 包装: antibody small pack of 25 μL
• 環境により配慮した代替製品の特徴: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 強化検証: recombinant expression; Learn more about Antibody Enhanced Validation
• テクニック: affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• アイソタイプ: IgG
• エピトープ配列: N-terminal half
• タンパク質IDアクセッション番号: NP_001955
• UniProtアクセッション番号: P18146
• 環境により配慮した代替製品カテゴリ: , Aligned
• 輸送温度: ambient
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... EGR1(1958)

詳細

詳細

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 1F22 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Early growth response protein 1 (EGR1). It targets an epitope within 23 amino acids from the N-terminal half.

免疫原

KLH-conjugated linear peptide corresponding to 23 amino acids from the N-terminal half of human Early growth response protein 1 (EGR1).

アプリケーション

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected EGR1 in HeLa cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected EGR1 in PC3 cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected EGR1 in human prostate tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected EGR1 in PC3 cells.

Affinity Binding Assay: A representative lot of this antibody bound EGR1 with a KD of 1.3 x 10-8 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

ターゲットの説明

Early growth response protein 1 (UniProt: P18146; also known as EGR-1, AT225, Nerve growth factor-induced protein A, NGFI-A, Transcription factor ETR103, Transcription factor Zif268, Zinc finger protein 225, Zinc finger protein Krox-24) is encoded by the EGR1 (also known as KROX24, ZNF225) gene (Gene ID: 1958) in human. EGR1 is a member of the EGR C2H2-type zinc-finger protein family that serves as a transcriptional regulator. It recognizes and binds to the DNA sequence 5′-GCG(T/G)GGGCG-3′(EGR-site) in the promoter region of target genes and regulates their transcription. It contains three C2H2-type Zinc fingers (a 338-362, 368-390, 396-418). The first, most N-terminal zinc finger binds to the 3′-GCG motif, the middle zinc finger interacts with the central TGG motif, and the C-terminal zinc finger binds to the 5′-GCG motif. It is shown to binds double-stranded target DNA, irrespective of the cytosine methylation status. It displays reduced affinity for target DNA where cytosines have been oxidized to 5-hydroxymethylcytosine and does not bind target DNA where cytosines have been oxidized to 5-formylcytosine or 5-carboxylcytosine. EGR1 plays an important role in regulating the response to growth factors, DNA damage, and ischemia. It is reported to activate the expression of p53/TP53 and TGFB1, and thereby prevents tumor formation and is shown to be essential for normal progress through mitosis and normal proliferation of hepatocytes after partial hepatectomy. It is also regulates the expression of proteins such as IL-1b and CXCL2 that are involved in inflammatory processes and development of tissue damage after ischemia. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zandarashvili, L., et al. (2015). FEBS Lett. 589(15); 1748-1753; Hashimoto, H., et al. (2014). Genes Dev. 28(20); 2304-2313).

物理的形状

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

再構成

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

保管および安定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法的情報

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 13 - Non Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable