ZRB1334

Anti-Clusterin Antibody, clone 2F23 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• UNSPSCコード: 12352203

特性

• 由来生物: rabbit
• 品質水準: 200
• リコンビナント: expressed in HEK 293 cells
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 2F23, recombinant monoclonal
• 詳細: recombinant, expressed in HEK 293 cells
• 製品種目: ZooMAb^® learn more
• 形状: lyophilized
• 分子量: calculated mol wt 52.50 kDa; observed mol wt ~60 and 38 kDa
• 化学種の反応性: human
• 包装: antibody small pack of 25 μL
• 環境により配慮した代替製品の特徴: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 強化検証: recombinant expression; Learn more about Antibody Enhanced Validation
• テクニック: flow cytometry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• アイソタイプ: IgG
• エピトープ配列: C-terminus
• タンパク質IDアクセッション番号: NP_001822
• UniProtアクセッション番号: P10909
• 環境により配慮した代替製品カテゴリ: Aligned,
• 輸送温度: ambient
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特異性

Clone 2F23 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects human Clusterin . It targets an epitope within 16 amino acids from the C-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal region of human Clusterin .

アプリケーション

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Clusterin in HeLa cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Clusterin in A549 cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Clusterin in human tonsil tissue sections.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Clusterin in human tonsil tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Clusterin, clone 2F23 ZooMAb^®, Cat. No. ZRB1334, is a recombinant Rabbit monoclonal antibody that specifically targets Clusterin and is tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting..

ターゲットの説明

Clusterin (UniProt: P10909; also known as Aging-associated gene 4 protein, Apolipoprotein J, Apo-J, Complement cytolysis inhibitor, CLI, Complement-associated protein SP-40,40, Ku70-binding protein 1, NA1/NA2, Sulfated glycoprotein 2, SGP-2, Testosterone-repressed prostate message 2, TRPM-2) is encoded by the CLU (also known as APOJ, CLI, KUB1, AAG4) gene (Gene ID: 1191) in human. Clusterin is a ubiquitously expressed protein that functions as extracellular chaperone that prevents aggregation of non-native proteins. It also prevents stress-induced aggregation of plasma proteins. Clusterin is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off to generate the mature form that can be proteolytically cleaved into a b-chain (aa 23-227) and an a-chain (aa 228-449). However, proteolytic cleavage is not necessary for its chaperone activity. Six different isoforms of Clusterin have been described that are produced by alternative splicing. Isoform 1 is shown to retro-translocate from the secretory compartments to the cytosol upon cellular stress, but isoforms 4 and 6 retain their cytoplasmic localization in stressed and unstressed cell. It maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70, but does not refold proteins by itself. Clusterin is reported to bind to Amyloid beta peptides and alters their aggregation and promote their clearance, suggesting its neuroprotective role. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Foster, EM., et al. (2019). Front. Neurosci. 13; 64; Rohne, P., et al. (2014). Cell Physiol. Biochem. 34(5); 1626-1639; Amy R Wyatt, AR., and Wilson, MR. (2010). J. Biol. Chem. 285(6); 3532-3539; Poon, S., et al. (2000). Biochemistry. 39(51); 15953-15960).

物理的形状

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

再構成

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

保管および安定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法的情報

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable