ZMS1072

Anti-p16 Antibody, clone D25 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• リコンビナント: expressed in HEK 293 cells
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: D25, recombinant monoclonal
• 製品種目: ZooMAb^® learn more
• 形状: lyophilized
• 分子量: calculated mol wt 16.53 kDa; observed mol wt ~16 kDa
• 化学種の反応性: human
• 包装: antibody small pack of 25 μL
• 環境により配慮した代替製品の特徴: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 強化検証: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• テクニック: affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; western blot: suitable
• アイソタイプ: IgG1
• エピトープ配列: Unknown
• UniProtアクセッション番号: P42771
• 環境により配慮した代替製品カテゴリ: , Aligned
• 輸送温度: ambient
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特異性

Clone D25 is a ZooMAb^® mouse recombinant monoclonal antibody that specifically detects Cyclin-dependent kinase inhibitor 2A (p16).

免疫原

Recombinant human p16 fusion protein.

アプリケーション

Quality Control Testing

Evaluated by Western Blotting with human recombinant p16 (CDKN2A)..

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected human recombinant p16 (CDKN2A).

Tested applications

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected p16 in HeLa cells.
Flow Cytometry Analysis: 1 μg from a representative lot detected p16 in Hela cells.

Affinity Binding Assay: A representative lot of this antibody bound recombinant human p16 (CDKN2A) with a KD of 2.5 x 10-6 in an affinity binding assay.

Flow Cytometry Analysis: 1 μg from a representative lot detected p16 (CDKN2A) in one million HeLa cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-p16, clone D25 ZooMAb^®, Cat. No. ZMS1072, is a recombinant Mouse monoclonal antibody that detects p16 (CDKN2A) and is tested for use in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, and Western Blotting.

ターゲットの説明

Cyclin-dependent kinase inhibitor 2A (UniProt: P42771; also known as Cyclin-dependent kinase 4 inhibitor A, CDK4I, Multiple tumor suppressor 1, MTS-1, p16-INK4a, p16-INK4, p16INK4A) is encoded by the CDKN2A (also known as CDKN2, MTS1) gene (Gene ID: 1029) in human. p16 serves as a negative regulator of cell proliferation by strongly interacting with CDK4 and CDK6 during the G1-to S-phase transition and blocking the phosphorylation of retinoblastoma (Rb) protein. Hence, it is considered as a tumor suppressor protein. Hypo-phosphorylated Rb protein forms complexes with E2F family of transcription factors, which sequesters E2Fs and prevent their access to the promoters of proliferation-associated genes. During cell cycle, in the late G1 phase CDK4 and CDK6 hyper-phosphorylate Rb protein that commits cells to enter into S phase. Binding of p16 maintains Rb in a hypo-phosphorylated state. CDKN2A gene is reported to be one of the most frequently mutated genes and is reported to be inactivated in about 50% of all human cancers. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Li, J., et al. (2011). Biochemistry. 50(25); 5566-5582; Sherr, CJ., and Roberts, JM (2004). Genes Dev. 18(22); 2699-2711; Ortega, S., et al. (2002). Biochim. Biophys. Acta. 1602(1); 73-87).

物理的形状

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

再構成

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

保管および安定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法的情報

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable