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Merck

Z0377

Sigma-Aldrich

Anti-Zyxin antibody, Mouse monoclonal

clone ZOL301, purified from hybridoma cell culture

別名:

Anti-ESP-2, Anti-HED-2

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About This Item

MDL番号:
UNSPSCコード:
12352203
NACRES:
NA.41

由来生物

mouse

品質水準

結合体

unconjugated

抗体製品の状態

purified immunoglobulin

抗体製品タイプ

primary antibodies

クローン

ZOL301, monoclonal

フォーム

buffered aqueous solution

分子量

antigen 82-84 kDa

交差性

mouse, rat, human

包装

antibody small pack of 25 μL

テクニック

immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1-2 μg/mL using total cell extract of HeLa cells.

アイソタイプ

IgG2a

UniProtアクセッション番号

輸送温度

dry ice

保管温度

−20°C

ターゲットの翻訳後修飾

unmodified

遺伝子情報

human ... ZYX(7791)
mouse ... Zyx(22793)
rat ... Zyx(114636)

詳細

Anti-Zyxin antibody, Mouse monoclonal (mouse IgG2a isotype) is derived from the hybridoma ZOL301 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to human Zyxin conjugated to KLH via an N-terminal added cysteine. Zyxin is a relatively low abundance component of focal adhesion plaques, stress fibers, and dynamic cell membrane areas such as the leading edge. It may also be found in the cell nucleus. Zyxin is a member of the LIM domain protein family that also includes LPP (Lipoma preferred partner), Trip6 (thyroid hormone interacting protein 6), and Ajuba. It is a phosphoprotein and comprises two major domains, an extended N-terminal domain that contains proline-rich sequence, and a C-terminal domain that contains a functional nuclear export signal and three double zinc-finger cysteine and histidine-rich LIM domains.

アプリケーション

Anti-Zyxin antibody, Mouse has been used in:
  • Western blotting
  • Immunofluorescence staining
  • Enzyme linked immunosorbent assay (ELISA)
  • Immunocytochemistry

生物化学的/生理学的作用

Zyxin interacts with multiple cytoskeletal, signaling and other proteins such as α -actinin, vasodilator-stimulated phosphoprotein (VASP), Mena, LIM-only cysteine-rich proteins (CRP1, CRP2, CRP3/MLP), Vav, breast cancer anti-estrogen resistance protein 1 (p130Cas), CRK-associated substrate-related protein/ (Human Enhancer of Filamentation 1) (CasL/HEF1, and serine-arginine(SR)-related protein (SON) DNA-binding protein. During mitosis a fraction of zyxin was reported to associate with the tumor suppressor h-warts/LATS1 (large tumor suppressor kinase 1) on the mitotic apparatus. Zyxin has been implicated in the regulation and modulation of actin filaments organization, cell adhesion, cell motility, mitosis, and signal transduction. Zyxin may shuttle between focal adhesion plaques and the nucleus. Involvement of zyxin in gene transcription by its association with transcription factors in the nucleus has been suggested.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

10 - Combustible liquids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable

個人用保護具 (PPE)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

Z0377-BULK:
Z0377-200UL:
Z0377-VAR:
Z0377-25UL:


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試験成績書(COA)

Lot/Batch Number

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以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Genetic ablation of zyxin causes Mena/VASP mislocalization, increased motility, and deficits in actin remodeling
Hoffman LM, et al.
The Journal of Cell Biology, 172(5), 771-782 (2006)
Zyxin, a regulator of actin filament assembly, targets the mitotic apparatus by interacting with h-warts/LATS1 tumor suppressor
Hirota T, et al.
The Journal of Cell Biology, 149(5), 1073-1086 (2000)
Zyxin and paxillin proteins: focal adhesion plaque LIM domain proteins go nuclear
Wang Y and Gilmore TD
Biochimica et Biophysica Acta - Molecular Cell Research, 1593(2-3), 115-120 (2003)
Identification of intracellular proteins and signaling pathways in human endothelial cells regulated by angiotensin-(1-7)
Meinert C, et al.
Journal of Proteomics, 130, 129-139 (2016)
Alexandra Woychek et al.
Cytoskeleton (Hoboken, N.J.), 76(2), 200-208 (2019-01-23)
The nuclear envelope protein nesprin-2G is a component of the linker of nucleoskeleton and cytoskeleton (LINC) complex and is responsible for mechanical and signaling crosstalk between the nucleus and cytoskeleton. A prior study has demonstrated that nesprin-2G knockout mice show

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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