S9555

Anti-E-Selectin (CD62E) antibody, Mouse monoclonal

clone 1.2B6, purified from hybridoma cell culture

• 別名: Monoclonal Anti-CD62E, Anti-E-Selectin, Anti-ELAM-1
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 1.2B6, monoclonal
• フォーム: buffered aqueous solution
• 分子量: antigen 100-115 kDa
• 化学種の反応性: human, pig
• 濃度: ~2 mg/mL
• テクニック: capture ELISA: suitable; flow cytometry: suitable; immunohistochemistry (frozen sections): suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; indirect immunofluorescence: 1-2 μg/mL using using 293-T cells transfected with E-selectin fusion protein; western blot: suitable (non-reduced)
• アイソタイプ: IgG1
• UniProtアクセッション番号: P16581
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... SELE(6401)

詳細

詳細

Monoclonal anti-E-selectin (CD62E) (mouse IgG1 isotype) is derived from the 1.2B6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with TNF-α activated human endothelial cells. This antibody reacts specifically with E-selectin (CD62E) expressed on the surface of activated endothelial cells. The antibody may weakly cross-react with recombinant human P-selectin and human platelet P-selectin. Cross-reactivity has been observed with porcine E-selectin.
It is a 140KDa protein mainly expressed in endothelial cells. E- selectin is characterized with an N-terminal domain, epidermal growth factor (EGF)-like domain, lectin (ligand-binding) domain, variable short consensus repeats and transmembrane domain.

Selectin E (SELE) is encoded by the gene mapped to human chromosome 1q24.2. The encoded protein is mainly expressed on luminal surfaces of inflamed vasculature.

特異性

Reacts specifically with human CD62E (E-Selectin, ELAM-1) expressed on the surface of activated endothelial cells. The antibody may weakly cross-react with recombinant hman P-Selectin and human platelet P-Selectin. Cross-reactivity has been observed with porcine E-Selectin. Monoclonal Anti-human E-Selctin inhibits adhesion of neutrophils, eosinophils, and a skin-homing T-cell subpopulation to activated endothelial cells and CD62E-transfected cells. 5th Workshop: code no. E 040.

免疫原

TNF-α activated human endothelial cells.

アプリケーション

Anti-E-Selectin (CD62E) antibody, Mouse monoclonal has been used in immunofluorescence.

生物化学的／生理学的作用

E-selectin acts as a key inflammatory cytokine. E-selectin-dependent rolling helps in binding pancreatic cancer cell to hyaluronic acid. In addition, the encoded protein in association with P-selectins, plays a vital role in recruiting leukocytes to inflammatory sites. Mutation in the gene is associated with the development of various diseases such as atherosclerosis and stroke.

Selectins play a vital role in maintaining homoeostasis and cutaneous inflammation. SEL-E and SEL-P help in carrying T helper (Th1) cells into inflamed tissues. SEL-E acts as a receptor for cluster of differentiation 44 (CD44 (HCELL)), Mac-2 binding protein, mucin 1 (Muc-1), death receptor-3 and E-selectin ligand-1 (ESL-1). Overexpression of the gene might affect adhesion and migration of the cells leading to the endometriosis disorder.

ターゲットの説明

CD62E (E-Selectin) surface antigen is a glycoprotein and member of the selectin family of adhesion receptors. It is expressed by activated human endothelial cells and is detectable in the plasma.

物理的形状

Solution, 0.2 μm filtered, in 0.01 M phosphate buffered saline, pH 7.4.

保管および安定性

For continuous use, store sterile at 2-8°C. For extended storage freeze in sterile working aliquots. Repeated freezing and thawing, or storage in "frostfree" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable