YEAST1

酵母トランスフェクションキット

reagents for introducing plasmid DNA into yeast

• 別名: lithium acetate yeast transformation
• UNSPSCコード: 12352200
• NACRES: NA.85

特性

• グレード: for molecular biology
• 品質水準: 200
• 使用法: kit sufficient for >100 standard transformations
• テクニック: transformation: suitable
• 輸送温度: dry ice
• 保管温度: −20°C

詳細

詳細

Sigma′s Yeast Transformation Kit contains all necessary reagents and controls for efficient transformation of yeast by the lithium acetate method.

アプリケーション

Suitable for transformation of any strain of yeast. Convenient, flexible and sensitive, positive transformants can be obtained with as little as 10 ng of DNA; the optimum efficiency is in the 0.1- 3 μg range.

特徴および利点

• そのまま使用可能です。
• 必要なプラスミドDNAはわずか10 ngです。
• どのような酵母株にも対応できます。

構成

The Yeast Transformation Kit contains:

• Transformation Buffer; 100 ml; 100 mM lithium acetate, 10 mM Tris HCl, pH 7.6, and 1 mM EDTA
• Plate Buffer; 100 ml; 40% PEG, 100 mM lithium acetate, 10 mM Tris HCl, pH 7.5, 1 mM EDTA
• Deoxyribonucleic acid from salmon teste, 10 mg/ml; 2 x 1 ml
• Control Yeast Plasmid DNA pRS316 carrying the ura gene; 10 μg
• Yeast Synthetic Drop-out Medium Supplement Without Uracil; 1 g

原理

Transformation with a plasmid complementing the mutated gene enables the transformant to grow on medium lacking the required component. Yeast cells are made competent for transformation by incubation in a buffered lithium acetate solution. Transformation is then carried out by incubating the cells together with transforming DNA and carrier DNA in a solution containing polyethylene glycol (PEG).

キットの構成要素は別途購入可能です。

• D9156 Deoxyribonucleic acid, single stranded from salmon testes, For hybridization 2 x 1
• Y1501 Yeast Synthetic Drop-out Medium Supplements, without uracil 1 g

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable