SRP3160

sRank レセプター ヒト

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• 別名: ODAR (骨芽細胞分化および活性化レセプター), TNFRSF11A, TRANCE レセプター
• UNSPSCコード: 12352200
• NACRES: NA.32

特性

• 由来生物: human
• リコンビナント: expressed in E. coli
• アッセイ: ≥98% (HPLC); ≥98% (SDS-PAGE)
• 形状: lyophilized
• 有効性: 30-50 ng/mL ED₅₀
• 分子量: 19.3 kDa
• 包装: pkg of 100 μg
• テクニック: cell culture | mammalian: suitable
• 不純物: <0.1 EU/μg endotoxin, tested
• 色: white
• UniProtアクセッション番号: Q9Y6Q6
• 輸送温度: wet ice
• 保管温度: −20°C
• 遺伝子情報: human ... TNFRSF11A(8792)

詳細

詳細

Soluble receptor activator of nuclear factor-κB (sRANK) also known as TNF receptor superfamily member 11a (TNFRSF11A), is encoded by the gene mapped to human chromosome 18. The encoded protein belongs to the tumor necrosis factor (TNF) receptor superfamily. RANK is widely expressed in variety of tissues, including skeletal muscle, thymus, liver, colon, small intestine, adrenal gland, osteoclast, mammary gland epithelial cells, prostate and pancreas. Recombinant human sRANK receptor is a 19.3kDa polypeptide containing the TNFR homologous cysteine rich portion of the extracellular domain of RANK receptor (175 amino acid residues).

生物化学的／生理学的作用

Soluble receptor activator of nuclear factor-κB (sRANK) plays a vital role in bone remodeling and fracture repair. Alterations in the RANK signaling increase the risk of susceptibility to expansile osteolysis and Paget disease of bone (PDB2). In addition, mutation of the gene coding for RANK/RANKL/OPG has also been observed in various skeletal disorders such as osteoporosis, glucocorticoid-induced bone loss, multiple myeloma, and rheumatoid arthritis. Binding of RANKL to its receptor RANK triggers the formation and differentiation of osteoclasts with the help of various transcription factors involved in modulation of osteoclastogenesis. RANK/RANKL/OPG might act as a potent therapeutic target for bone diseases.

シーケンス

MQIAPPCTSE KHYEHLGRCC NKCEPGKYMS SKCTTTSDSV CLPCGPDEYL DSWNEEDKCL LHKVCDTGKA LVAVVAGNST TPRRCACTAG YHWSQDCECC RRNTECAPGL GAQHPLQLNK DTVCKPCLAG YFSDAFSSTD KCRPWTNCTF LGKRVEHHGT EKSDAVCSSS LPARK

物理的形状

凍結乾燥品(10 mMリン酸ナトリウム、pH 7.2)。

再構成

バイアルは、開封前に遠心してください。0.1～1.0 mg/mLの濃度となるよう水で再構成してください。ボルテックスは行わないでください。この溶液は2～8°Cで1週間まで保存できます。長期保存の際は、キャリアタンパク質(0.1% BSAなど)を含有するバッファーで希釈し、1回使用量ごとに分けて-20～-80°Cで保存することをお勧めします。

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable