SRP3158

Sox2 TAT ヒト

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

• UNSPSCコード: 12352202
• NACRES: NA.32

特性

• 由来生物: human
• リコンビナント: expressed in E. coli
• アッセイ: ≥95% (HPLC); ≥95% (SDS-PAGE)
• 形状: lyophilized
• 分子量: 36 kDa
• 包装: pkg of 25 μg
• テクニック: cell culture | mammalian: suitable
• 不純物: <0.1 EU/μg endotoxin, tested
• 色: white
• UniProtアクセッション番号: P48431
• 輸送温度: wet ice
• 保管温度: −20°C
• 遺伝子情報: human ... SOX2(6657)

詳細

詳細

Sox2, also known as sex determining region Y (SRY)-box 2, is part of a diverse family of structurally-related transcription factors whose primary structure contains a 79-residue DNA-binding domain, called high mobility group (HMG) box. It contains 317 amino acids. The gene encoding this protein is localized on human chromosome 3q26.33. Recombinant human Sox2-TAT expressed in E. coli is a 36kDa protein containing 330 amino-acid residues, including the 317 residues of full-length Sox2 and a 13-residue C-terminal TAT peptide (GGYGRKKRRQRRR).

生物化学的／生理学的作用

Sex determining region Y (SRY)-box 2 (SRY-2) or Sox2 plays an essential role in maintaining the pluripotency of embryonic stem cells (ESC) and determination of cell fate. Sox2 acts as a transcriptional activator after forming a ternary complex with Oct3/4 and a conserved non-coding DNA sequence (CNS1) located approximately 2kb upstream of the RAX promoter. The protein modulates stem cell function in embryonic and neural stem cells. Microarray analysis showed that Sox2 regulates the expression of multiple genes involved in embryonic development including fibroblast growth factor-4 (FGF-4), YES1 (a tyrosine-protein kinase) and ZFP206 (a transcription factor). Sox2 and other transcription factors have been introduced into cells by DNA transfection, viral infection, or microinjection. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors and other nuclear proteins into primary as well as transformed cells.

シーケンス

MYNMMETELK PPGPQQTSGG GGGNSTAAAA GGNQKNSPDR VKRPMNAFMV WSRGQRRKMA QENPKMHNSE ISKRLGAEWK LLSETEKRPF IDEAKRLRAL HMKEHPDYKY RPRRKTKTLM KKDKYTLPGG LLAPGGNSMA SGVGVGAGLG AGVNQRMDSY AHMNGWSNGS YSMMQDQLGY PQHPGLNAHG AAQMQPMHRY DVSALQYNSM TSSQTYMNGS PTYSMSYSQQ GTPGMALGSM GSVVKSEASS SPPVVTSSSH SRAPCQAGDL RDMISMYLPG AEVPEPAAPS RLHMSQHYQS GPVPGTAING TLPLSHMGGY GRKKRRQRRR

物理的形状

凍結乾燥品(10 mMリン酸ナトリウム、pH 8.0)。

再構成

バイアルは、開封前に遠心してください。0.1～1.0 mg/mLの濃度となるよう水で再構成してください。ボルテックスは行わないでください。この溶液は2～8°Cで1週間まで保存できます。長期保存の際は、キャリアタンパク質(0.1% BSAなど)を含有するバッファーで希釈し、1回使用量ごとに分けて-20～-80°Cで保存することをお勧めします。

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable