SRP0242

EGFR (T790M L858R) Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

• 別名: ERBB1, HER1, PIG61
• UNSPSCコード: 12352200
• NACRES: NA.32

特性

• 由来生物: human
• リコンビナント: expressed in baculovirus infected insect cells
• アッセイ: ≥70% (SDS-PAGE)
• 形状: aqueous solution
• 分子量: 88 kDa
• 包装: pkg of 10 μg
• 保管条件: avoid repeated freeze/thaw cycles
• 濃度: >0.02 mg/mL
• NCBIアクセッション番号: NM_005228
• UniProtアクセッション番号: P00533
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... EGFR(1956)

詳細

詳細

Epidermal growth factor receptor (EGFR) can be activated by binding EGF or at least six other structurally related protein ligands, including transforming growth factor-α (TGF-α), heparin-binding EGF-like growth factor (HB-EGF), betacellulin (BTC), amphiregulin, epiregulin and epigen. Human EGFR T790M L858R (GenBank Accession No. NM_005228), (amino acids 668-1210) with N-terminal GST tag, MW = 88kDa, expressed in baculovirus expression system.
The EGFR gene is mapped to human chromosome 7p11.2 and encodes a 170 kDa receptor protein belonging to the HER (Receptor tyrosine-protein kinase erbB-4) family of receptor tyrosine kinases. Egfr is localized to epithelial cell surface.

アプリケーション

酵素キネティクスの研究をはじめ、インヒビターのスクリーニングや選択性プロファイリングにもご利用いただけます。

生物化学的／生理学的作用

Epidermal growth factor receptor (EGFR, ErbB1) is a transmembrane protein that exerts tyrosine kinase activity upon ligand induced activation. The receptor undergoes dimerization and phosphorylation of tyrosine kinase. Upon activation by ligands, epidermal growth factor receptor (EGFR, ErbB1) initiates a signaling cascade which includes dimerization and internalization, tyrosine phosphorylation, DNA synthesis of target genes, and, ultimately, cell proliferation. EGFR signaling plays a role in the growth and differentiation of normal cells, but elevated EGFR activity is correlated with the development and pathogenesis of certain cancers. Mutation in the EGFR gene is associated with the risk of tumor development including glioma, colorectal adenoma, colorectal, breast and non-small cell lung cancer.

単位の定義

1ユニットは、pH 7.4、30°Cで1分あたり1 pmolのチロシン基質をリン酸化する酵素量と定義されています。

物理的形状

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

調製ノート

氷上で解凍してください。最初に解凍した際、チューブを軽く遠心して内容物を完全に回収してください。酵素液は一回の使用量ずつ分注してください。分注した酵素液は希釈せずそのまま-70°Cで保存してください。注:酵素は凍結融解の繰り返しに対して非常に不安定です。