SBR00024

Trimethoprim Ready Made Solution

25 mg/mL in DMSO

• 別名: Trimethoprim Ready Made Solution
• 実験式（ヒル表記法）: C₁₄H₁₈N₄O₃
• 分子量: 290.32
• UNSPSCコード: 51285203
• NACRES: NA.76

特性

• 由来生物: synthetic
• アッセイ: ≥98% (HPLC)
• 形状: liquid
• 濃度: 25 mg/mL in DMSO
• 色: colorless to faint yellow
• 抗生物質活性スペクトル: Gram-negative bacteria; Gram-positive bacteria; mycobacteria
• 作用機序: DNA synthesis | interferes; enzyme | inhibits
• 保管温度: −20°C
• InChI: 1S/C14H18N4O3/c1-19-10-5-8(6-11(20-2)12(10)21-3)4-9-7-17-14(16)18-13(9)15/h5-7H,4H2,1-3H3,(H4,15,16,17,18
• InChI Key: IEDVJHCEMCRBQM-UHFFFAOYSA-N

詳細

詳細

Trimethoprim, an aminopyrimidine antibiotic belonging to the methoxybenzenes group, exhibits activity against a broad spectrum of both gram-negative and gram-positive aerobic bacteria. As a synthetic derivative of pyrimidine, it plays a multifaceted role as an inhibitor, xenobiotic, and allergen, featuring antibacterial, diuretic, and antiprotozoal properties. Trimethoprim selectively targets dihydrofolate reductase (DHFR), diminishing the levels of tetrahydrofolate that supply one-carbon units crucial for biosynthesis processes, including nucleotides, proteins, and panthotenate, especially in prokaryotic microorganisms.

Additionally, it is frequently employed in combination with sulfamethoxazole (sc-208405) to effectively inhibit the growth of Staphylococcus aureus, functioning as a dihydrofolate reductase inhibitor. This versatile compound finds applications in metabolomics, cell biology, and biochemical research.

アプリケーション

Trimethoprim is used at a final concentration of ~4μg/mL for antibacterial activty.

生物化学的／生理学的作用

Mode of Action: Trimethoprim is an inhibitor of bacterial dihydrofolate reductase (DHFR), interfering with the conversion of dihydrofolate (DHF) to tetrahydrofolate (THF). THF is an essential precursor in the thymidine synthesis pathway, interference with this pathway inhibits bacterial DNA synthesis. Trimethoprim′s affinity for bacterial DHFR is several thousand times greater than its affinity for human DHFR.

Activity Spectrum: Effective against Gram-negative bacteria, Gram-positive bacteria, and mycobacteria

Some of the pathogens that trimethoprim can be effective against include:

• Escherichia coli (E. coli)
• Staphylococcus aureus (including methicillin-resistant Staphylococcus aureus or MRSA)
• Streptococcus pneumoniae
• Haemophilus influenzae
• Enterococcus faecalis
• Klebsiella species
• Proteus species

特徴および利点

• High-quality antibiotic suitable for multiple research applications
• Ideal for Cell Biology, Metabolomics, and Biochemical research.

その他情報

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安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): 188.6 °F
• 引火点(℃): 87 °C