おすすめの製品
詳細
SARS-CoV-2 belongs to Coronaviridae (CoVs) - a large family of viruses that usually cause mild to moderate upper-respiratory tract diseases. CoVs are single-stranded RNA viruses. They have a high mutation rate and are therefore highly diverse. Their hosts include human and non-human mammals, as well as birds. This product is expressed in human HEK 293 cells as a glycoprotein with a calculated molecular mass of 25 kDa (amino acids 319- 541) and includes a C-terminal polyhistidine tag. The DTT-reduced protein migrates as a ~35 kDa polypeptide on SDS-PAGE because of glycosylation. This protein is produced in human cells, without the use of serum. The human cells expression system allows human like glycosylation and folding, and often supports higher specific binding of the protein.
This Spike protein RBD is labeled with biotin, to form biotin-tagged protein. This Spike protein RBD biotin-tagged may serve as a useful tool for binding experiments while detecting protein:protein interaction assays using binding partners such as ACE2. The degree of biotinylation is >=90%. This Spike protein RBD-biotin can be visualized with streptavidin-conjugated probes (e.g., Streptavidin-HRP conjugate).
This Spike protein RBD is labeled with biotin, to form biotin-tagged protein. This Spike protein RBD biotin-tagged may serve as a useful tool for binding experiments while detecting protein:protein interaction assays using binding partners such as ACE2. The degree of biotinylation is >=90%. This Spike protein RBD-biotin can be visualized with streptavidin-conjugated probes (e.g., Streptavidin-HRP conjugate).
アプリケーション
The activity of this product is tested by a functional ELISA. Immobilization of ACE2 (Cat. No. SAE0064) at 2 μg/mL (100 μL/well), allows binding of Spike protein RBD, biotin tagged. The half maximal binding (binding which produces 50% of the optimal binding response) is = 2 µg/mL.
Purity: =95% (SDS-PAGE)
Purity: =95% (SDS-PAGE)
- Exploration of the protein protein interaction of CoVs Spike protein with is partners, like ACE2 protein.
- Tool for screening inhibitors for the interaction of CoVs Spike protein with ACE2.
生物化学的／生理学的作用
SARS-CoV-2 infects cells which express angiotensin converting enzyme 2 (ACE2). ACE2 binds to the viral envelope-associated spike (S) protein as a cellular receptor, following proteolytic cleavage of both S and ACE2 by serine proteases. S protein is believed to be the target of neutralizing antibodies because it is the main trans-membrane glycoprotein responsible for receptor-binding and virion entry.
The S protein is a trimeric protein. During the binding to ACE2, S protein undergoes a substantial structural rearrangement to facilitate the fusion of the viral membrane with the host cell membrane. The receptor binding domain (RBD) of S protein is the domain that specifically binds to ACE2. RBD is the major target for neutralizing antibodies for coronaviruses, and anti-RBD antibodies likely correlate with virus neutralization.The ACE2:spike interaction has a high affinity of 15 nM.
The S protein is a trimeric protein. During the binding to ACE2, S protein undergoes a substantial structural rearrangement to facilitate the fusion of the viral membrane with the host cell membrane. The receptor binding domain (RBD) of S protein is the domain that specifically binds to ACE2. RBD is the major target for neutralizing antibodies for coronaviruses, and anti-RBD antibodies likely correlate with virus neutralization.The ACE2:spike interaction has a high affinity of 15 nM.
保管分類コード
11 - Combustible Solids
WGK
WGK 1
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
SAE0199-50UG:
SAE0199-BULK:
SAE0199-VAR:
最新バージョンのいずれかを選択してください：
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）