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由来生物
alpaca
リコンビナント
expressed in E. coli
結合体
unconjugated (6*his tag and one cys were added at the C terminal of the VHH)
抗体製品の状態
affinity purified immunoglobulin
抗体製品タイプ
secondary antibodies
クローン
recombinant monoclonal
形状
lyophilized solid
分子量
14 kDa
化学種の反応性
Alpaca, mouse
テクニック
ELISA: 1:10000-1:50000
western blot: 1:10000-1:50000
サイズ
0.1 mL
色
colorless
アイソタイプ
VHH
輸送温度
wet ice
保管温度
2-8°C
詳細
Alpaca Anti-Mouse IgG2b(Fcγ) single domain Antibody is designed to specifically detect mouse IgG2b Fcγ fragment. It is a monovalent, single domain, recombinant antibody derived from the variable region of heavy chain of Alpaca pacous. Based on immunoelectrophoresis and/or ELISA, Alpaca Anti-Mouse IgG2b(Fcγ) single domain Antibody reacts with the Fc fragment of mouse IgG2b selectively but not with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins.
Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. Mouse IgG constitutes 75% of serum immunoglobulins, and IgG is the predominant form of first antibody produced from mouse. Mouse IgG consists of five subclasses-IgG1, IgG2a, IgG2b, IgG2c(inbred mouse strains with the Igh1-b allele have IgG2c isotype instead of IgG2a), IgG3. They are highly homologous and differ mainly in the hinge region. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains, and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. Mouse IgG constitutes 75% of serum immunoglobulins, and IgG is the predominant form of first antibody produced from mouse. Mouse IgG consists of five subclasses-IgG1, IgG2a, IgG2b, IgG2c(inbred mouse strains with the Igh1-b allele have IgG2c isotype instead of IgG2a), IgG3. They are highly homologous and differ mainly in the hinge region. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains, and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
特異性
Mouse IgG2b (Fcγ fragment specific)
Isotype: VHH domain of alpaca IgG2b/2c
Isotype: VHH domain of alpaca IgG2b/2c
免疫原
Recombinant Alpaca single variable domain secondary Nanobody
Recombinant Alpaca single variable domain secondary nano antibody
包装
100 μg in Glass bottle
物理的形状
Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose
再構成
Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL
保管および安定性
After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Stable for 12 months at -20°C
その他情報
No cross-reactivity with mouse IgG1/2a/3, mouse IgM, rabbit, human, cynomolgus, rat, goat IgG
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シグナルワード
Warning
危険有害性情報
危険有害性の分類
Aquatic Chronic 3 - Skin Sens. 1
保管分類コード
13 - Non Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
SAB5801009-100UG:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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