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由来生物
mouse
結合体
unconjugated
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
2H7, monoclonal
形状
buffered aqueous solution
化学種の反応性
nonhuman primates, human
濃度
1 mg/mL
テクニック
flow cytometry: 0.6 μg/mL
アイソタイプ
IgG2b
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
保管温度
2-8°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... MS4A1(931)
詳細
CD20 is a cell surface 33-37 (depending on the degree of phosphorylation) kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells (low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells). Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. It is associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment.
The CD20 gene, mapped to human chromosome 11q12-13, codes for a B lymphocyte differentiation antigen. The protein encoded by the gene is a 33kDa transmembrane phosphoprotein, specifically expressed in normal and neoplastic B-cells. CD20, also known as MS4A1, is a member of membrane-spanning 4-domains subfamily A (MS4A) family of molecules with multiple membrane spanning domains.
特異性
The antibody 2H7 reacts with CD20 (Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on B lymphocytes (it is lost on plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes.
免疫原
Human tonsillar B cells
アプリケーション
The reagent is designed for Flow Cytometry analysis. Suggested working dilution for Flow Cytometry is 0.6 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
生物化学的／生理学的作用
Restricted expression pattern of CD20 in B- cells helps in distinguishing B-cell from T-cell lymphomas. CD20, is considered to be a potential therapeutic target in the treatment of B-cell malignancies such as diffuse large B-cell lymphoma and chronic lymphocytic leukemia. CD20, might act as an ion channel, thereby permitting the influx of calcium essential for B cell activation, proliferation and differentiation. In humans CD20 deficiency, leads to impaired T cell–independent antibody responses. The monoclonal anti-CD20 antibody, Rituximab (RTX) reduces the increased CD20 expressing T cells in multiple sclerosis patients.
特徴および利点
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
物理的形状
PBS（pH 7.4、15 mM アジ化ナトリウム含有）
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
SAB4700114-100UG:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Katrin A Salva et al.
The American Journal of dermatopathology, 37(10), e116-e121 (2015-09-19)
Expression of the pan B-cell marker CD20 by T-cell lymphoproliferative disorders is exceedingly rare. We present a 52-year-old man with a unilesional cutaneous CD20 T-cell lymphoproliferative disorder. Multispectral imaging analysis of CD3-CD20 double-stained lesional tissue sections allowed (1) the visualization
Taco W Kuijpers et al.
The Journal of clinical investigation, 120(1), 214-222 (2009-12-30)
CD20 was the first B cell differentiation antigen identified, and CD20-specific mAbs are commonly used for the treatment of B cell malignancies and autoantibody-mediated autoimmune diseases. Despite this the role of CD20 in human B cell physiology has remained elusive.
Arumugam Palanichamy et al.
Journal of immunology (Baltimore, Md. : 1950), 193(2), 580-586 (2014-06-15)
In multiple sclerosis (MS), B cell-depleting therapy using monoclonal anti-CD20 Abs, including rituximab (RTX) and ocrelizumab, effectively reduces disease activity. Based on indirect evidence, it is generally believed that elimination of the Ag-presenting capabilities and Ag nonspecific immune functions of
T F Tedder et al.
Journal of immunology (Baltimore, Md. : 1950), 142(7), 2555-2559 (1989-04-01)
The human CD20 gene (B1) encodes a B lymphocyte-specific, cell-surface molecule that is involved in B cell activation and differentiation. We report that the CD20 gene is located on human chromosome 11 at position q12-q13. The location of CD20 was
Jessica L Teeling et al.
Journal of immunology (Baltimore, Md. : 1950), 177(1), 362-371 (2006-06-21)
We have previously defined a panel of fully human CD20 mAb. Most of these were unexpectedly efficient in their ability to recruit C1q to the surface of CD20-positive cells and mediate tumor lysis via activation of the classical pathway of
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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