SAB4200842

Anti-PMEL antibody, Mouse monoclonal

clone PL-19, purified from hybridoma cell culture

• UNSPSCコード: 12352203
• NACRES: NA.41
• クローン: PL-19
• application: IF
• 化学種の反応性: human
• テクニック: immunoblotting: 1-2 μg/mL using human melanoma SK-MEL-28 cell lysate; immunofluorescence: 1-2 μg/mL using human melanoma SK-MEL-28 cells
• citations: 6

特性

• 抗体製品の状態: purified from hybridoma cell culture
• 品質水準: 200
• 抗体製品タイプ: primary antibodies
• クローン: PL-19
• フォーム: liquid
• 化学種の反応性: human
• 濃度: ~1 mg/mL
• テクニック: immunoblotting: 1-2 μg/mL using human melanoma SK-MEL-28 cell lysate; immunofluorescence: 1-2 μg/mL using human melanoma SK-MEL-28 cells
• アイソタイプ: IgG1
• UniProtアクセッション番号: P40967
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PMEL(6490)

詳細

詳細

The melanocyte-specific protein PMEL (also known as Pmel17, gp100, melanocyte protein, premelanosome protein or silver locus protein homolog (SILV) is a type I transmembrane glycoprotein that is expressed primarily in pigment cells of the eye and skin. In melanosomes, PMEL protein forms a fibrillar matrix on which the UV-shielding pigment melanin is deposited.1-2

特異性

Monoclonal Anti-PMEL antibody specifically recognizes human PMEL protein.

アプリケーション

The antibody may be used in various immunochemical techniques including Immunoblotting (~100 kDa), Immunohistochemistry and Immunofluorescence.

生物化学的／生理学的作用

PMEL fibrils are a major functional component of the melanosomal compartment as they optimize the melanin polymerization, condensation and storage.1,3
In addition, PMEL fibrils have an amyloidogenic nature sharing features with pathological amyloids.4 Thus, PMEL is suggested as an excellent model system to study mechanisms of intracellular amyloid formation.1
Mutations in PMEL are associated with pigmentation disorders and/or impairments in eye development in various species.1,5,6

物理的形状

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

保管および安定性

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免責事項

Unless otherwise stated in our catalog  our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable