SAB4200559

Anti-VAChT (C-terminal) antibody produced in rabbit

IgG fraction of antiserum

• 別名: Anti-SLC18A3, Anti-Solute carrier family 18 member 3, Anti-VAT, Anti-vesicular acetylcholine transporter
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: IgG fraction of antiserum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 交差性: rat, mouse, human
• テクニック: indirect immunofluorescence: 1:1,000-1:2,000 using PC12 cells treated with rat NGF.; western blot: 1:500-1:800 using extracts of mouse brain (S1 fraction).
• UniProtアクセッション番号: Q16572
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... SLC18A3(6572) ; mouse ... slc18a3(20508) ; rat ... slc18a3(60422)

詳細

詳細

Vesicular Acetylcholine Transporter (VAChT, SLC18A3) belongs to the solute carrier family 18 (SLC18) family of proton/neurotransmitter antiporters and shares a high degree of homology to vesicular monoamine transporters (VMATs). It is a 12-transmembrane domain protein. VAChT is expressed in all major cholinergic neurons in the central and peripheral nervous system and is mainly localized in small synaptic vesicles in cholinergic nerve terminals.

免疫原

synthetic peptide corresponding to a sequence at the C-terminus of human vesicular acetylcholine transporter (VAChT), conjugated to KLH. The corresponding sequence is highly conserved in rat and mouse VAChT (82% identity).

アプリケーション

Anti-VAChT (C-terminal) antibody produced in rabbit has been used in:

• immunoblotting
• immunofluorescence staining
• immunocytochemistry
• double immunolabeling

生物化学的／生理学的作用

Vesicular Acetylcholine Transporter (VAChT), is required in cholinergic neurons for the selective transport of acetylcholine (ACh) into synaptic vesicles. It depends on the activity of a vacuolar-type H⁺ -ATPase to accumulate ACh in synaptic vesicles. Knockdown of VAChT in the hippocampus has been shown to cause behavior changes, learning deficits, specific deficits in spatial memory and long-term potentiation (LTP). VAChT provides a specific marker for cholinergic neurons, for the study of cholinergic transmission in experimental models of Alzheimer′s disease and other nervous system disorders. Cloning of the VAChT gene has revealed a single genetic locus encoding both VAChT and ChAT, providing a unique genomic arrangement suggesting that these genes may be co-regulated.

物理的形状

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable