SAB4200548

Anti-IDH1 (R132H) antibody, Mouse monoclonal

clone HMab-1, purified from hybridoma cell culture

• 別名: 抗IDCD抗体, 抗IDH抗体, 抗IDPC抗体, 抗IDP抗体, 抗PICD抗体, 抗可溶性イソクエン酸デヒドロゲナーゼ1(NADP+)抗体
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: HMab-1, monoclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~43 kDa
• 化学種の反応性: human
• 濃度: ~1.0 mg/mL
• テクニック: western blot: 4.0-8.0 μg/mL using extract of HEK-293T cells overexpressing IDH1^R132H.
• UniProtアクセッション番号: O75874
• アプリケーション: research pathology
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... IDH1(3417)

詳細

詳細

Anti-IDH1 (R132H) antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma HMab1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide corresponding to mutation R132H of human IDH1. A member of isocitrate dehydrogenase (IDH) family, IDH1, is the human cytoplasmic NADP-specific enzyme. Its subcellular localization was shown to be in both peroxisomes and the cytoplasm.

免疫原

peptide corresponding to mutation R132H of human IDH1.

アプリケーション

Anti-IDH1 (R132H) antibody has been used:

• in immunoblotting
• in immunohistochemistry
• in western blotting
• in cell cycle analysis and apoptosis assays
• in chromatin immunoprecipitation (ChIP) assay
• in in vitro migration assay

生物化学的／生理学的作用

Isocitrate dehydrogenase 1 (IDH1) catalyzes the oxidative decarboxylation of isocitrate into α α -KG) using NADP as co-substrate. Mutations in IDH1 are specific to Arg132 (R132) and endow them with the function of generating 2-hydroxyglutarate (2HG) instead of α-KG. This product alters gene transcription through effects on DNA and histone methylation. Several IDH1 mutations exist, including R132H, R132C, R132S, R132G and R132L. Each may result in different tumor type with varied malignant progression. The most frequent known mutation (>90%) is the alteration of arginine to histidine (R132H). Hence, antibodies that recognize the IDH1^R132H mutation can be useful for the diagnosis of mutation-bearing tumors like gliomas.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable