SAB4200527

Anti-USP8 antibody, Mouse monoclonal

clone US872, purified from hybridoma cell culture

• 別名: 抗hUBPy抗体, 抗ユビキチンC末端ヒドロラーゼ8抗体, 抗ユビキチンチオエステラーゼ8抗体, 抗ユビキチン特異的プロセシングプロテアーゼ8抗体, 抗脱ユビキチン化酵素8抗体
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: US872, monoclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~127 kDa; antigen ~70 kDa
• 化学種の反応性: human, rat, bovine, monkey, mouse, dog
• 濃度: ~1.0 mg/mL
• テクニック: flow cytometry: 5-10 μg/test using MCF7 cells; indirect immunofluorescence: 1.25-2.5 μg/mL using A549 cells; western blot: 0.5-1.0 μg/mL using MCF7 total cell extracts
• UniProtアクセッション番号: P40818
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... USP8(9101)

詳細

詳細

Monoclonal Anti-USP8 (mouse IgG1 isotype) is derived from the hybridoma US872 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Ubiquitin specific protease 8 (USP8) belongs to the family of de-ubiquitinating enzymes (DUBs).

免疫原

synthetic peptide corresponding to an internal sequence of human USP8, conjugated to KLH.

アプリケーション

Monoclonal Anti-USP8 antibody produced in mouse has been used in:immunoblotting immunofluorescence,flow cytometry, immunocytochemistry.

生物化学的／生理学的作用

Ubiquitin specific protease 8 (USP8) was found to regulate ubiquitination of frizzled, a receptor for Wnt/Wingless. Deletion of USP8 in mice causes embryonic lethality. The defect was accompanied by a strong reduction or absence of several growth factor receptor tyrosine kinases (RTKs), like EGFR, hepatocyte growth factor receptor (c-met), and ERBB3.USP8 inactivation results in enhanced ubiquitination of ligand-activated EGFR. USP8 in conjunction with components of the ESCRT-0 complex, plays an integral role in the early endosomal sorting machinery that functions to protect epidermal growth factor receptor (EGFR) as well as other receptors from lysosomal degradation.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable