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Merck

SAB4200497

Sigma-Aldrich

Anti-THY1 antibody, Mouse monoclonal

clone TH350, purified from hybridoma cell culture

別名:

Anti-CD90, Anti-CDw90, Anti-FLJ33325, Anti-Thy-1 T-cell antigen, Anti-Thy-1 antigen, Anti-Thy-1 cell surface antigen, Anti-thy-1 membrane glycoprotein, Monoclonal Anti-THY1 antibody produced in mouse

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About This Item

UNSPSCコード:
12352203
NACRES:
NA.41

由来生物

mouse

結合体

unconjugated

抗体製品の状態

purified from hybridoma cell culture

抗体製品タイプ

primary antibodies

クローン

TH350, monoclonal

形状

buffered aqueous solution

分子量

antigen ~27 kDa

化学種の反応性

mouse, human, rat

濃度

~1.0 mg/mL

テクニック

ELISA: suitable
flow cytometry: 20-40 μg/test using A549 cells.
immunohistochemistry: suitable
western blot: 2.0-4.0 μg/mL using NT2 total cell extracts.

UniProtアクセッション番号

輸送温度

dry ice

保管温度

−20°C

ターゲットの翻訳後修飾

unmodified

遺伝子情報

human ... THY1(7070)

詳細

Monoclonal Anti-THY1 (mouse IgG1 isotype) is derived from the hybridoma TH350 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the N-terminus of human THY1, conjugated to keyhole limpet hemocyanin (KLH). Monoclonal Anti-THY1 recognizes human, rat and mouse Thy-1.
Thy-1 cell surface antigen (THY1) is a glycoprotein that is expressed on the membranes of adult cells, mesenchymal stromal cells and cancer cells. It has a molecular weight of about 25-37kDa. The gene encoding THY1 is localized on human chromosome 11q23.3.
THY-1 is expressed in various cell types, including human fibroblasts, neurons, blood stem cells and endothelial cells as well as murine T cells.

特異性

Monoclonal Anti-THY1 recognizes human, rat and mouse Thy-1.

免疫原

synthetic peptide corresponding to a sequence at the N-terminus of human THY1, conjugated to KLH.

アプリケーション

Monoclonal Anti-THY1 antibody produced in mouse may be used in:
  • immunoblotting
  • flow cytometry
  • immunofluorescence

生物化学的/生理学的作用

THY-1 is also involved in the inhibition of neurite outgrowth, apoptotic signaling, leukocyte and melanoma cell adhesion and migration and fibroblast proliferation and migration. This protein plays a role as a mediator of cell-cell and cell-matrix interactions. Thy-1 is a cell surface marker in identifying local and circulating liver cancer stem cells. This protein′s expression can be regulated by the epigenetic mechanism of promoter region methylation, in which down-regulation of Thy-1 is associated with a more invasive/metastatic clinical phenotype.
Thy-1 cell surface antigen (THY1) has a role in the activation of T-cells, release of neurotransmitters at synapses, enhancement of adhesion of astrocytes, wound healing, fibrogenesis and tumor suppression. It also plays an important role in cell migration and cytoskeletal reorganization.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

保管および安定性

For extended storage, freeze at -20 0C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

10 - Combustible liquids

WGK

WGK 2

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

SAB4200497-200UL:
SAB4200497-BULK:
SAB4200497-VAR:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Thomas H Barker et al.
Experimental cell research, 295(2), 488-496 (2004-04-20)
The precise biological role of Thy-1, a glycophosphatidyl-inositol (GPI)-linked cell surface glycoprotein in non-caveolar lipid raft microdomains, remains enigmatic. Evidence suggests that Thy-1 affects intracellular signaling through src-family protein kinases, and modulates adhesive and migratory events, such as thymocyte adhesion
Physical linkage of the fragile site FRA11B and a Jacobsen Syndrome chromosome deletion breakpoint in 11q23. 3
Jones C, et al.
Human Molecular Genetics, 3, 2123-2130 (1994)
A reduction in CD90 (THY-1) expression results in increased differentiation of mesenchymal stromal cells.
Moraes DA, et al.
Stem Cell Research & Therapy, 7 (2016)
Zhen Fan Yang et al.
Cancer cell, 13(2), 153-166 (2008-02-05)
This study characterized cancer stem cells (CSCs) in hepatocellular carcinoma (HCC) cell lines, tumor specimens, and blood samples. The CD90+ cells, but not the CD90(-) cells, from HCC cell lines displayed tumorigenic capacity. All the tumor specimens and 91.6% of
Tanya A Rege et al.
Biochimica et biophysica acta, 1763(10), 991-999 (2006-09-26)
Thy-1 is a 25-37 kDa glycosylphosphatidylinositol (GPI)-anchored protein involved in T cell activation, neurite outgrowth, apoptosis, tumor suppression, wound healing, and fibrosis. To mediate these diverse effects, Thy-1 participates in multiple signaling cascades. In this review, we discuss Thy-1 signaling

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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