SAB4200473

Anti-EpCAM ICD antibody, Mouse monoclonal

clone 4A7, purified from hybridoma cell culture

• 別名: モノクローナル抗TACSTD1抗体
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: 4A7, monoclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~40 kDa
• 化学種の反応性: human, mouse
• 濃度: ~1.0 mg/mL
• テクニック: indirect immunofluorescence: 5-10 μg/mL using human MCF7 cells.; western blot: 0.25-0.5 μg/mL using whole extracts of human SW480 cells.
• アイソタイプ: IgG1
• UniProtアクセッション番号: P16422
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... EPCAM(4072) ; mouse ... Epcam(17075)

詳細

詳細

Monoclonal Anti- EpCAM ICD (mouse IgG1 isotype) is derived from the hybridoma 4A7 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the intracellular domain of human EpCAM, conjugated to KLH. Epithelial cell adhesion molecule (EpCAM) is an epithelial transmembrane glycoprotein, composed of an ectodomain, a transmembrane domain and a cytoplasmic domain. EpCAM is frequently expressed in human malignancies, normal stem and progenitor cells, and at lower levels, in normal epithelia.

免疫原

synthetic peptide corresponding to the intracellular domain of human EpCAM, conjugated to KLH. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

アプリケーション

Monoclonal Anti-EpCAM ICD antibody has been used in

• immunoblotting
• immunoprecipitation
• immunofluorescence

生物化学的／生理学的作用

Epithelial cell adhesion molecule′s (EpCAM) intracellular domain (ICD) fragment translocates to the nucleus after cleavage from EpCAM ectodomain and associates with other molecules to bind DNA resulting in cell proliferation and tumor formation.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable