SAB4200365

Anti-PIWIL1 antibody, Mouse monoclonal

clone PIWIL1-2, purified from hybridoma cell culture

• 別名: Monoclonal Anti-PIWIL1 antibody produced in mouse, Monoclonal Anti-HIWI, Monoclonal Anti-MIWI, Monoclonal Anti-PIWI, Monoclonal Anti-piwi-like 1
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: PIWIL1-2, monoclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~95 kDa
• 化学種の反応性: human, mouse
• 濃度: ~1.0 mg/mL
• テクニック: immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded mouse testis and biotin / ExtrAvidin^® Peroxidase staining system.; immunoprecipitation (IP): suitable; western blot: 2.5-5.0 μg/mL using whole cell extracts of HEK-293T cells over-expressing PIWIL1.
• アイソタイプ: IgG2b
• UniProtアクセッション番号: Q96J94
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PIWIL1(9271) ; mouse ... Piwil1(57749)

詳細

詳細

Monoclonal Anti-PIWIL1 (mouse IgG2b isotype) is derived from the hybridoma piwi-like protein 1 (PIWIL1-2) produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal region of human PIWIL1 conjugated to KLH. PIWIL1 is a member of Piwi subfamily. It is expressed mostly to the germline. The encoded protein consists of the PAZ domain and the PIWI domain. The PIWIL1 gene is located on human chromosome 12q24.33.

免疫原

synthetic peptide corresponding to an internal region of human PIWIL1, conjugated to KLH. The corresponding sequence differs by one amino acid in mouse PIWIL1.

アプリケーション

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)

Monoclonal Anti-PIWIL1 antibody produced in mouse has been used in:

• immunofluorescence
• immunoprecipitation
• flow cytometry analysis29}
• immunoblotting
• immunohistochemistry

生物化学的／生理学的作用

Piwi proteins are implicated in epigenetic control of gene expression, transposon silencing and translation regulation. It plays vital roles in the germline development and gametogenesis of many metazoan species.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

法的情報

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable