SAB4200248

モノクローナル抗アクチン抗体 マウス宿主抗体

clone MM2/193, purified from hybridoma cell culture

• MDL番号: MFCD00145889
• UNSPSCコード: 12352203
• NACRES: NA.41
• 結合体: unconjugated
• application: WB
• クローン: MM2/193, monoclonal
• 化学種の反応性: mouse, human, rat, canine, monkey
• citations: 13
• テクニック: western blot: 1-2 μg/mL using HeLa cell extracts

特性

• 由来生物: mouse
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: MM2/193, monoclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~42 kDa
• 化学種の反応性: mouse, human, rat, canine, monkey
• 濃度: ~1.0 mg/mL
• テクニック: western blot: 1-2 μg/mL using HeLa cell extracts
• アイソタイプ: IgM
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... ACTB(60)

詳細

詳細

Monoclonal anti-actin (mouse IgM isotype) is derived from the hybridoma MM2/193 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human platelet membranes. Monoclonal anti-actin recognizes human, monkey, dog, rat and mouse actin (not tested in other species).
In mammals, actin protein consists of six different isoforms encoded by separate genes. The four isoforms α_skeletal-actin, α _cardiac-actin, α_smooth-actin and γ_smooth-actin are expressed in skeletal, cardiac and smooth muscle. The other two isoforms, β cyto-actin and γ cyto-actin are ubiquitously expressed.

免疫原

hybridoma MM2/193 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human platelet membranes

アプリケーション

Monoclonal Anti-Actin antibody produced in mouse has been used in immunoblotting.

生物化学的／生理学的作用

Actin plays a vital role in various cellular processes, such as cell division, migration, junction formation, chromatin remodeling, transcriptional regulation, vesicle trafficking and cell shape regulation. Polymorphism in the smooth muscle alpha-actin (ACTA2) is associated with the development of thoracic aortic aneurysms and dissections.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

保管および安定性

Store at -20°C. For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20°C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

その他情報

In order to obtain the best results using various techniques and preparations, we recommend determining optimal working dilutions by titration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable