SAB4200141

抗Rictor抗体 ウサギ宿主抗体

~1-2 μg/mL, affinity isolated antibody

• 別名: ANTI-Pianissimo, ANTI-Rapamycin-insensitive companion of mTOR, ANTI-TORC2-specific protein AVO3, Anti-RPTOR independent companion of MTOR, complex 2, AVO3
• UNSPSCコード: 12352203
• NACRES: NA.44

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: ~190 kDa
• 化学種の反応性: human, rat, mouse
• 包装: antibody small pack of 25 μL
• 濃度: ~1-2 μg/mL
• テクニック: immunohistochemistry: 5-10 μg/mL using a working concentration of 5-10 ug/mL is recommended using heat-retrieved formalin-fixed, paraffin-embedded rat testis sections and biotin / ExtrAvidin Peroxidase staining system.; immunoprecipitation (IP): 5-10 μg using a working amount of 5-10 μg is recommended using lysates of human HeLa cells.; western blot: 1-2 μg/mL using a working concentration of 1-2 μg/mL is recommended using whole extracts of mouse C2C12 cells.
• UniProtアクセッション番号: Q6R327
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... RICTOR(253260) ; mouse ... rictor(78757) ; rat ... rictor(310131)

詳細

詳細

Rictor (rapamycin-insensitive companion of mTOR) is a subunit of the mammalian target of rapamycin complex 2 (mTORC2). The mTORC2 complex contains mTOR, mLST8, mSIN1, deptor, protor-1 and rictor. mTOR substrate that regulates cell size.

m-TOR is a conserved kinase that primarily regulates cell growth. Rictor is a rapamycin-insensitive companion of m-TOR and regulates the phosphorylation of Akt/PKB. Rictor also phosphorylates PKCα and actin cytoskeleton .

特異性

Anti-Rictor antibody is specific for human, mouse and rat rictor. In immunoblotting, detection of the rictor band (approx. 190 kDa) is specifically inhibited by the immunizing peptide.

免疫原

The corresponding sequence is identical in mouse and rat rictor.

アプリケーション

Anti-Rictor antibody is suitable for use in immunohistochemistry (5-10 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded rat testis sections and biotin/ExtrAvidin Peroxidase staining system). The product may also be used for immunoprecipitation (5-10 μg using lysates of human HeLa cells), and immunoblot (1-2 μg/mL is using whole extracts of mouse C2C12 cells).

生物化学的／生理学的作用

Rictor (rapamycin-insensitive companion of mTOR) regulates cell survival and cytoskeletal organization through the regulation of Akt/ protein kinase B (PKB) and protein kinase C alpha (PKCα).

物理的形状

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable