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由来生物
rat
結合体
unconjugated
抗体製品の状態
purified from hybridoma cell culture
抗体製品タイプ
primary antibodies
クローン
monoclonal
フォーム
buffered aqueous solution
分子量
antigen ~270 kDa
化学種の反応性
rat, human
包装
antibody small pack of 25 μL
テクニック
western blot: 2-4 μg/mL using using Jurkat cell extracts
アイソタイプ
IgG2a
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... NOTCH3(4854)
rat ... Notch3(56761)
詳細
Monoclonal Anti-Notch3 (rat IgG2a isotype) is derived from the hybridoma NIZ 8G5 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from the immunized rat.
Members of the Notch gene family encode transmembrane receptors. Mammals have four Notch receptors, Notch1 through Notch4. Notch1 and Notch2 have the highest homology with each other, whereas Notch3 and Notch4 are structurally divergent, lacking the transactivation domain that is found in Notch1 and Notch2. The Notch3 receptor possesses 34 extracellular epidermal growth factor (EGF)-like repeats and three cysteine-rich Notch/LIN12 repeats (LIN). It also contains two protein-protein interaction domains, the RAM domain (R), two nuclear localization signals (NLS), six ankyrin (ANK) repeats and a PEST sequence (P) at its cytoplasmic portion. The NOTCH3 gene is mapped to human chromosome 19p13.12.
Members of the Notch gene family encode transmembrane receptors. Mammals have four Notch receptors, Notch1 through Notch4. Notch1 and Notch2 have the highest homology with each other, whereas Notch3 and Notch4 are structurally divergent, lacking the transactivation domain that is found in Notch1 and Notch2. The Notch3 receptor possesses 34 extracellular epidermal growth factor (EGF)-like repeats and three cysteine-rich Notch/LIN12 repeats (LIN). It also contains two protein-protein interaction domains, the RAM domain (R), two nuclear localization signals (NLS), six ankyrin (ANK) repeats and a PEST sequence (P) at its cytoplasmic portion. The NOTCH3 gene is mapped to human chromosome 19p13.12.
特異性
Monoclonal Anti-Notch3 recognizes human and rat Notch3.
免疫原
human recombinant full length Notch3.
アプリケーション
Monoclonal Anti-Notch3 antibody produced in rat is suitable for immunoblotting.
生物化学的／生理学的作用
The Notch receptors are critical for various cell fate decisions. The Notch signaling plays a key role in the normal development of many tissues and cell types. It has diverse effects on differentiation, survival and/or proliferation that are highly dependent on the signal strength and cellular context. Variations in the NOTCH3 gene leads to vascular degenerative disease known as cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). In addition, upregulation of the NOTCH3 gene indicates active and relapsing human T-cell acute lymphoblastic leukemia (T-ALL).
物理的形状
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
保管および安定性
Store at -20 °C. For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20 °Cin working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discardedif not used within 12 hours.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
SAB4200125-200UL:
SAB4200125-VAR:
SAB4200125-25UL:
SAB4200125-BULK:
最新バージョンのいずれかを選択してください：
Silvia Bianchi et al.
Journal of cellular physiology, 207(2), 300-308 (2005-11-05)
Notch proteins encode a family of transmembrane receptors that are part of a signalling transduction system known as Notch signalling, an extremely conserved and widely used mechanism regulating programs governing growth, apoptosis and differentiation in metazoans. Notch signalling begins when
N Turner et al.
Oncogene, 29(14), 2013-2023 (2010-01-27)
Triple negative breast cancers (TNBCs) have a relatively poor prognosis and cannot be effectively treated with current targeted therapies. We searched for genes that have the potential to be therapeutic targets by identifying genes consistently overexpressed when amplified. Fifty-six TNBCs
Charlotte E Vorwald et al.
Journal of molecular medicine (Berlin, Germany), 98(3), 425-435 (2020-02-06)
Cell-based therapeutic approaches are an exciting strategy to replenish compromised endothelial cell (EC) populations that contribute to impaired vasculogenesis. Co-cultures of ECs and mesenchymal stromal cells (MSCs) can enhance neovascularization over ECs alone, but the efficacy of cells is limited
Aušra Sasnauskienė et al.
Medicina (Kaunas, Lithuania), 50(1), 14-18 (2014-07-26)
Notch signaling is a conserved developmental pathway, which plays an important role in the regulation of cell proliferation, differentiation and death. Deregulation of Notch pathway has been connected with the carcinogenesis in a variety of cancers. The aim of this
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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