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Merck

SAB4200112

Sigma-Aldrich

Anti-EIF2C3/Ago3 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

別名:

Anti-Argonaute 3, Anti-Eukaryotic translation initiation factor 2C, subunit 3

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About This Item

UNSPSCコード:
12352203
NACRES:
NA.41

由来生物

rabbit

結合体

unconjugated

抗体製品の状態

affinity isolated antibody

抗体製品タイプ

primary antibodies

クローン

polyclonal

形状

buffered aqueous solution

分子量

antigen ~97 kDa

化学種の反応性

human

濃度

~1.0 mg/mL

テクニック

immunoprecipitation (IP): 2.5-5 μg using lysates of HEK-293T cells over expressing human EIF2C3/Ago3
indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde fixed HEK-293T cells over expressing human EIF2C3/Ago3
western blot: 0.5-1 μg/mL using lysates of HEK-293T cells over expressing human EIF2C3/Ago3

輸送温度

dry ice

保管温度

−20°C

ターゲットの翻訳後修飾

unmodified

遺伝子情報

human ... EIF2C3(192669)

詳細

Anti-eukaryotic translation initiation factor 2C 3 (EIF2C3)/protein argonaute-3 (Ago3) is developed in rabbit using as immunogen a synthetic peptide corresponding to human EIF2C3/Ago3 conjugated to KLH. Argonaute proteins (molecular mass 100 kDa) are characterized by piwi-argonaute-zwille (PAZ) and PIWI domains. Ago subfamily consists of four members, hsAgo1-4. hsAgo2 and Ago3 associate primarily with miRNA. Ago proteins localize to the cytoplasm of somatic cells and are concentrated in cytoplasmic processing bodies. A member of this group, Ago1, is associated with the Golgi and endoplasmic reticulum. The gene is located on chromosome 1 in a cluster of closely related family members including Ago 1 and Ago 4.
Argonaute 3, RISC catalytic component (Ago3), also known as eukaryotic translation initiation factor 2C, 3 (EIF2C3), is encoded by the gene mapped to human chromosome 1p34.3. The encoded protein belongs to the argonaute family of proteins and is characterized with a PAZ (Piwi/Argonaute/Zwille) domain and PIWI domains.

免疫原

synthetic peptide corresponding to amino acids 13-25 of human EIF2C3/Ago3 conjugated to KLH. The corresponding sequence differs by one or two amino acid in rat and mouse, respectively.

アプリケーション

Anti-EIF2C3/Ago3 antibody produced in rabbit has been used in immunoprecipitation and western blot assays.
Anti-EIF2C3/Ago3 antibody produced in rabbit has been used:
  • immunoprecipitation
  • immunoblotting
  • immunofluorescence

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

生物化学的/生理学的作用

Argonaute proteins play a vital role in RNA interference (RNAi). These proteins are also involved in germline development, stem cell fate determination, as well as epigenetic regulation and transposon silencing. Ago3 facilitates the regulation of let-7a-3p expression. Mutation in the gene might lead to the development of Wilms′ tumors.
hAgo2 has been shown to have slicing activity. This region is lost in Wilms′ tumors, which are hypothesized to be caused by defects in embryonic kidney development, which disturb the capacity of metanephrogenic precursor cells to differentiate.

物理的形状

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

10 - Combustible liquids

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

SAB4200112-VAR:
SAB4200112-200UL:
SAB4200112-BULK:
SAB4200112-25UL:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

[The functional analysis of polymorphic insertions of Alu retroelements at acute lymphoblastic leukemia].
Komkov Aiu
Bioorganicheskaia khimiia, 38, 351-364 (2012)
PAPI, a novel TUDOR-domain protein, complexes with AGO3, ME31B and TRAL in the nuage to silence transposition
Liu L
Development, 138, 1863-1873 (2011)
Hypoxia potentiates microRNA-mediated gene silencing through posttranslational modification of Argonaute2
Wu C, et al.
Molecular and Cellular Biology, 31(23), 4760-4774 (2011)
The Argonaute family: tentacles that reach into RNAi, developmental control, stem cell maintenance, and tumorigenesis
Carmell MA
Genes & Development, 16, 2733-2742 (2002)
eIF1A augments Ago2-mediated Dicer-independent miRNA biogenesis and RNA interference
Yi T
Nature Communications, 6 (2015)

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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